| Urea transport in initial IMCD of rats fed a low-protein diet: functional properties and mRNA abundance. | |
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MedLine Citation:
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PMID: 7611462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Feeding rats a low-protein (8%) diet (LPD) for 2 wk induces a facilitated urea transporter in rat initial inner medullary collecting ducts (IMCDs). To determine whether this is preceded by an increase in mRNA abundance, we designed degenerate polymerase chain reaction primers to the rabbit facilitated urea transporter (UT2; G. You, C. P. Smith, Y. Kanai, W.-S. Lee, M. Stelzner, and M. A. Hediger. Nature Lond. 365: 844-847, 1993) and amplified a 716-bp cDNA fragment to perform Northern analysis of the base or tip of rat inner medulla. In the base, the predominant transcript was a 2.9-kb band, which increased 55% after 1 wk on an LPD; there was no change in a 4-kb band. In the tip, the 4-kb band predominated, but neither band varied with an LPD. Next, we functionally characterized the induced urea transporter using microperfused initial IMCDs from rats fed an LPD for 2 wk. First, 100 pM arginine vasopressin (AVP) stimulated urea permeability (Purea); 10 nM AVP increased Purea further. Second, raising perfusate and bath osmolality to 690 mosmol/kgH2O (NaCl added) stimulated Purea; adding AVP (10 nM) increased Purea further. Third, thiourea reversibly inhibited AVP-stimulated Purea.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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Z M Ashkar; S Martial; T Isozaki; S R Price; J M Sands |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 268 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-08-14 Completed Date: 1995-08-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F1218-23 Citation Subset: IM |
Affiliation:
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Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U10358 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Arginine / pharmacology Base Sequence Biological Transport Blotting, Northern Carrier Proteins / biosynthesis*, metabolism Diet, Protein-Restricted* Gene Expression* / drug effects Kidney Medulla / drug effects, metabolism*, physiology Kidney Tubules, Collecting / drug effects, metabolism*, physiology Male Membrane Glycoproteins / biosynthesis*, metabolism Membrane Transport Proteins* Molecular Sequence Data Osmolar Concentration Polymerase Chain Reaction RNA, Messenger / analysis, biosynthesis, metabolism* Rabbits Rats Rats, Sprague-Dawley Thiourea / pharmacology Urea / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01-DK-45688/DK/NIDDK NIH HHS; R29-DK-41707/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/RNA, Messenger; 0/urea transporter; 57-13-6/Urea; 62-56-6/Thiourea; 74-79-3/Arginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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