Document Detail


Uptake and utilization of arachidonic acid in infective larvae of Angiostrongylus cantonensis.
MedLine Citation:
PMID:  21106133     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Infective larvae of Angiostrongylus cantonensis may take up and incorporate exogenous arachidonic acid into their lipid pool. By scintillation counting, uptake and incorporation were determined to be time dependent. Arachidonic acid was mainly incorporated into phospholipid (56.8%) and neutral lipid (22.4%) pools. In the neutral lipids, 64.0% was diglyceride and 36.0% triglyceride. Phosphatidylcholine was the predominant fatty acid in the phospholipid pool. In addition to the release of leukotriene B4, the parasite was found to generate radiolabelled CO2 after incubation with [U-14C]arachidonate. Moreover, enzymatic analysis of crude extracts revealed the presence of acyl-CoA dehydrogenase (short and long chain), thiolase, enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase. These findings suggest that infective larvae of A. cantonensis not only take up and incorporate exogenous arachidonic acid into their lipid pool, but may also utilize the fatty acid through a functional β-oxidation pathway.
Authors:
P Tang; L-C Wang
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Publication Detail:
Type:  Journal Article     Date:  2010-11-25
Journal Detail:
Title:  Journal of helminthology     Volume:  85     ISSN:  1475-2697     ISO Abbreviation:  J. Helminthol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985115R     Medline TA:  J Helminthol     Country:  England    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
Affiliation:
Department of Parasitology, College of Medicine, Chang Gung University, Tao-Yuan 33332, Taiwan.
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