Document Detail


Uptake of transmitter amino acids by glial plasmalemmal vesicles from different regions of rat central nervous system.
MedLine Citation:
PMID:  7824067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From rat hippocampal homogenate, we recently isolated a novel subcellular fraction richly containing glial plasmalemmal vesicles (GPV), which takes up glutamate remarkably as a synaptosomal fraction [Y. Nakamura et al. (1993) Glia, 9, 48-56]. In the present study, we prepared GPV from different regions of rat CNS, namely olfactory bulb (Ob), cerebral cortex (Cx), caudatoputamen (Cp), hippocampus (Hp), cerebellum (Ce) and spinal cord (Sc), and analyzed their activities of Na(+)-dependent uptake of following neurotransmitters and a related compound; glutamate, gamma-aminobutyrate (GABA), glycine, dopamine and choline. The uptake activities of these amino acids were not significantly different between GPV and synaptosomes in each region. Regionally, however, the activities were varied considerably. The activities of glutamate uptake revealed in the following rank order: Cx, Hp, Cp > Ce, Ob > Sc. GABA uptake activities were: Ce > Ob, Cx, Hp > Cp, while glycine uptake activities were: Sc, Ce > Ob, Cp, Cx, Hp. On the other hand, the uptake activities of dopamine and choline were quite different between GPV and synaptosomes. Synaptosomal fraction from Cp took up dopamine in a high activity; however, GPV from the same tissue hardly showed the uptake activity. Choline was taken up by synaptosomes prepared from Hp but not by GPV.
Authors:
Y Nakamura; H Kubo; K Kataoka
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  19     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-02-14     Completed Date:  1995-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1145-50     Citation Subset:  IM    
Affiliation:
Department of Physiology, Ehime University, School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / metabolism,  ultrastructure
Central Nervous System / metabolism*
Choline / metabolism
Dopamine / metabolism
Glutamic Acid / metabolism
Glycine / metabolism
Liposomes
Neuroglia / metabolism*,  ultrastructure
Neurotransmitter Agents / metabolism*
Rats
Rats, Wistar
gamma-Aminobutyric Acid / metabolism
Chemical
Reg. No./Substance:
0/Liposomes; 0/Neurotransmitter Agents; 56-12-2/gamma-Aminobutyric Acid; 56-40-6/Glycine; 56-86-0/Glutamic Acid; 62-49-7/Choline

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