Document Detail

Uptake of synthetic polynucleotides by competent cells of Bacillus subtilis.
MedLine Citation:
PMID:  4992366     Owner:  NLM     Status:  MEDLINE    
A survey was made of the capacity of competent cells of Bacillus subtilis to take up synthetic polynucleotides. Polydeoxyribonucleotides but not polyribonucleotides are taken up by the cells. Both types of polynucleotide failed to compete with transforming deoxyribonucleic acid in the transformation assay whereas both stimulated amino acid incorporation. The latter phenomenon appears to be nonspecific as there is no correlation between the codon composition of the polynucleotides employed and the amino acids whose incorporation was stimulated.
O Ciferri; S Barlati; J Lederberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  104     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1970 Nov 
Date Detail:
Created Date:  1971-02-09     Completed Date:  1971-02-09     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  684-8     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Bacillus subtilis / growth & development,  metabolism*
Bacteriological Techniques
Carbon Isotopes
Culture Media
DNA, Bacterial / metabolism
Genetics, Microbial*
Polynucleotides / metabolism*,  pharmacology
Time Factors
Transformation, Genetic* / drug effects
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Culture Media; 0/DNA, Bacterial; 0/Polynucleotides; 10028-17-8/Tritium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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