Document Detail

Uptake and remodeling of exogenous phosphatidylethanolamine in E. coli.
MedLine Citation:
PMID:  15164768     Owner:  NLM     Status:  MEDLINE    
The fate of exogenous short-chain analogues of phosphatidylethanolamine and phosphatidylserine was studied in a deep-rough derivative of E. coli mutant strain AD93 that cannot synthesize phosphatidylethanolamine de novo. Using mass spectrometry, it was shown that dicaproyl(di 6:0)-phosphatidylethanolamine is extensively remodeled, eventually adopting the phosphatidylethanolamine species profile of the parental wild-type strain of AD93. Dicaproyl-phosphatidylserine was decarboxylated to form phosphatidylethanolamine, and yielded a species profile, which strongly resembled that of the introduced phosphatidylethanolamine. This demonstrates transport of phosphatidylserine to the cytosolic leaflet of the inner membrane. The changes of the species profile of phosphatidylethanolamine indicate that the short-chain phospholipids are most likely remodeled via two consecutive acyl chain substitutions, and at least part of this remodeling involves transport to the inner membrane.
Matthijs A Kol; Diederik W D Kuster; Henry A Boumann; Hans de Cock; Albert J R Heck; Ben de Kruijff; Anton I P M de Kroon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1636     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-05-28     Completed Date:  2004-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  205-12     Citation Subset:  IM    
Department of Biochemistry of Membranes, Institute of Biomembranes, Ctr. Biomem. and Lipid Enz. (CBLE), Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
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MeSH Terms
Chromatography, Thin Layer
Electrophoresis, Polyacrylamide Gel
Escherichia coli / metabolism*
Mass Spectrometry
Phosphatidylethanolamines / metabolism*
Reg. No./Substance:
0/Phosphatidylethanolamines; 39382-08-6/phosphatidylethanolamine

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