Document Detail

Uptake and release of neurotransmitters.
MedLine Citation:
PMID:  18428535     Owner:  NLM     Status:  MEDLINE    
The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug interactions with transporter recognition sites using radioligands, as well as the characterization of drug effects on selective transporter-mediated uptake and release of substrate. In addition to clonal cell lines, synaptosomes prepared from specific brain regions can be used to conduct these studies without interference by endogenous transporters or binding proteins that are present in other tissues. This unit presents protocols for uptake and release of tritiated substrates using intact cells (either detached or in suspension) or synaptosomes. An HPLC procedure for electrochemical detection of nonradiolabeled substrates is also provided. Time-dependent release can also be measured in assays involving real-time sampling.
A Janowsky; K Neve; A J Eshleman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]     Volume:  Chapter 7     ISSN:  1934-8576     ISO Abbreviation:  Curr Protoc Neurosci     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706581     Medline TA:  Curr Protoc Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit7.9     Citation Subset:  IM    
Oregon Health Sciences University and Veterans Affairs Medical Center, Portland, Oregon, USA.
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MeSH Terms
Cell Line / drug effects,  metabolism
Chromatography, High Pressure Liquid / methods
Clone Cells / drug effects,  metabolism
Corpus Striatum / cytology,  drug effects,  metabolism
Dopamine / metabolism,  secretion
Dopamine Plasma Membrane Transport Proteins / chemistry,  genetics,  metabolism*
Neurotransmitter Agents / metabolism*,  secretion
Psychotropic Drugs / pharmacology
Radioligand Assay
Receptors, Dopamine / metabolism
Recombinant Fusion Proteins / chemistry,  metabolism
Synaptosomes / drug effects,  metabolism,  secretion
Reg. No./Substance:
0/Dopamine Plasma Membrane Transport Proteins; 0/Neurotransmitter Agents; 0/Psychotropic Drugs; 0/Receptors, Dopamine; 0/Recombinant Fusion Proteins

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