| Uptake and release of neurotransmitters. | |
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MedLine Citation:
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PMID: 18428535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug interactions with transporter recognition sites using radioligands, as well as the characterization of drug effects on selective transporter-mediated uptake and release of substrate. In addition to clonal cell lines, synaptosomes prepared from specific brain regions can be used to conduct these studies without interference by endogenous transporters or binding proteins that are present in other tissues. This unit presents protocols for uptake and release of tritiated substrates using intact cells (either detached or in suspension) or synaptosomes. An HPLC procedure for electrochemical detection of nonradiolabeled substrates is also provided. Time-dependent release can also be measured in assays involving real-time sampling. |
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Authors:
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A Janowsky; K Neve; A J Eshleman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] Volume: Chapter 7 ISSN: 1934-8576 ISO Abbreviation: Curr Protoc Neurosci Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-04-22 Completed Date: 2008-05-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706581 Medline TA: Curr Protoc Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: Unit7.9 Citation Subset: IM |
Affiliation:
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Oregon Health Sciences University and Veterans Affairs Medical Center, Portland, Oregon, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line / drug effects, metabolism Chromatography, High Pressure Liquid / methods Clone Cells / drug effects, metabolism Corpus Striatum / cytology, drug effects, metabolism Dopamine / metabolism, secretion Dopamine Plasma Membrane Transport Proteins / chemistry, genetics, metabolism* Electrochemistry Humans Kidney Neurotransmitter Agents / metabolism*, secretion Psychotropic Drugs / pharmacology Radioligand Assay Rats Receptors, Dopamine / metabolism Recombinant Fusion Proteins / chemistry, metabolism Synaptosomes / drug effects, metabolism, secretion |
| Chemical | |
Reg. No./Substance:
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0/Dopamine Plasma Membrane Transport Proteins; 0/Neurotransmitter Agents; 0/Psychotropic Drugs; 0/Receptors, Dopamine; 0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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