Document Detail


Uptake, release, and metabolism of alanine in neurons and astrocytes in primary cultures.
MedLine Citation:
PMID:  8377225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The uptake, release, and metabolism of alanine were studied in primary cultures of cerebral cortical neurons or astrocytes and cerebellar granule neurons. All three cell types exhibited a saturable, sodium-dependent uptake of alanine with Km values (microM) of 256 +/- 30, 463 +/- 39, and 292 +/- 39, respectively, and Vmax values (nmol/min/mg) of 15.9 +/- 0.7, 7.9 +/- 0.01, and 17.4 +/- 0.8, respectively. The corresponding values (nmol/min/mg) for the specific activity of alanine aminotransferase were 4.7 +/- 0.4, 17.1 +/- 2.5, and 4.5 +/- 0.9 (all values represent the mean +/- SEM). Release of alanine from the cells was rectilinear with time over a 10 hr period in case of astrocytes (40 nmol/hr/mg) and cerebellar granule neurons (21 nmol/hr/mg). In cortical neurons the release rate declined from an initial value of 19 nmol/hr/mg during the first 3 hr to a value of less than 3 nmol/hr/mg during the subsequent 7 hr of incubation. Metabolism of [14C]alanine to 14CO2 was found to have a lag period of 15 min and subsequently the rate of CO2 production was constant over a 45 min period with a value of 0.5 nmol/min/mg in granule neurons and about 0.3 nmol/min/mg in the other two cell types. Altogether the results show that alanine is preferentially produced in and released from astrocytes and accumulated into both GABAergic cortical neurons and glutamatergic cerebellar granule neurons.
Authors:
N Westergaard; T Varming; L Peng; U Sonnewald; L Hertz; A Schousboe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  35     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-10-21     Completed Date:  1993-10-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  540-5     Citation Subset:  IM    
Affiliation:
PharmaBiotec Research Center, Department of Biological Sciences, Royal Danish School of Pharmacy, Copenhagen.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism
Alanine / metabolism*
Alanine Transaminase / metabolism*
Amino Acids / pharmacology
Animals
Animals, Newborn
Astrocytes / metabolism*
Biological Transport / drug effects
Cerebellum / metabolism*
Cerebral Cortex / metabolism*
Embryo, Mammalian
Frontal Lobe / metabolism
Kinetics
Mice
Neurons / metabolism*
Tritium
Chemical
Reg. No./Substance:
0/Amino Acids; 10028-17-8/Tritium; 56-41-7/Alanine; EC 2.6.1.2/Alanine Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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