Document Detail

Uptake and release of bilirubin by skin.
MedLine Citation:
PMID:  4772626     Owner:  NLM     Status:  MEDLINE    
1. Skin epithelium of albino rat, mouse and guinea pig was shown to accumulate bilirubin from a medium containing free or bound bilirubin. 2. The K(m) values for bound bilirubin were 2.22x10(-3), 1.33x10(-3) and 9.5x10(-4)m for rat, mouse and guinea pig respectively and the corresponding K(m) values for free bilirubin were 5.2x10(-4), 4.0x10(-4), 1.8x10(-4)m; the V(max.) values of bound and free bilirubin were unchanged. 3. The uptake showed saturation kinetics. Bound bilirubin was released together with serum proteins. Free bilirubin bound to skin was not released into the medium. 4. Freezing and thawing of skin epithelium did not cause any significant lowering of the uptake of bilirubin but heat-denatured skin epidermis took up only 50% of the bound bilirubin or free bilirubin taken up by control unheated skin epithelium. 5. The uptake of free and bound bilirubin was prevented by HgCl(2) but not by sodium arsenate, NaCN, NaF, cycloheximide, 2,4-dinitrophenol or iodoacetate. 6. Most of the free bilirubin was bound to the lipids or lipoprotein fraction of skin epithelium and could be extracted by solvents. 7. Rat skin showed the highest accumulation and efflux of bilirubin.
C L Kapoor; C R Murti; P C Bajpai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  136     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Sep 
Date Detail:
Created Date:  1974-03-19     Completed Date:  1974-03-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  35-43     Citation Subset:  IM    
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MeSH Terms
Arsenic / pharmacology
Bilirubin / metabolism*
Biological Transport, Active / drug effects
Blood Proteins / metabolism
Cyanides / pharmacology
Cycloheximide / pharmacology
Depression, Chemical
Dinitrophenols / pharmacology
Epithelium / metabolism
Fluorides / pharmacology
Guinea Pigs
Hot Temperature
Iodoacetates / pharmacology
Lipid Metabolism
Lipoproteins / metabolism
Mercury / pharmacology
Protein Binding
Skin / drug effects,  metabolism*
Reg. No./Substance:
0/Blood Proteins; 0/Cyanides; 0/Dinitrophenols; 0/Fluorides; 0/Iodoacetates; 0/Lipoproteins; 635-65-4/Bilirubin; 66-81-9/Cycloheximide; 7439-97-6/Mercury; 7440-38-2/Arsenic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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