| Uptake and release of 2-aminoisobutyrate by Leishmania donovani: culture-age dependent effects of osmolality and of protein kinase inhibitors. | |
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MedLine Citation:
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PMID: 9004489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rate of uptake of 2-aminoisobutyrate (AIB), a non-metabolizable analogue of alanine, by Leishmania donovani was investigated as a function of culture age and osmolality in the presence of protein kinase inhibitors and of glucose, glutamate or proline. Hyperosmolality inhibited AIB uptake by cells of both growth stages, but to a greater extent for log than for stationary cells. Staurosporine, a protein kinase C inhibitor, had no effect on AIB uptake by cells of either culture age, but it reduced the rate of AIB release in response to hypo-osmolality, and more so in stationary cells than in log cells. Genistein, a protein-tyrosine kinase inhibitor, caused a small increase in AIB uptake by log cells under both iso- and hyperosmotic conditions, but had no effect on AIB uptake by stationary cells. Genistein also caused a small increase in the rate of release of AIB in response to a decrease in osmolality. Brief preincubation with glucose, glutamate or proline of cells that had been depleted of their osmolytes by prior exposure to hypo-osmolality caused an increase in AIB release by log cells, but a decrease by stationary cells. Similarly, whereas glucose and glutamate increased the rate of AIB uptake by log cells, and proline inhibited it, they had no effect on AIB uptake by stationary cells. Thus AIB uptake and release are sensitive to changes in osmolality, to protein kinase inhibitors, and to certain nutrients in a manner that changes markedly with culture age. |
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Authors:
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J J Blum |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of the cell / under the auspices of the European Cell Biology Organization Volume: 87 ISSN: 0248-4900 ISO Abbreviation: Biol. Cell Publication Date: 1996 |
Date Detail:
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Created Date: 1997-02-20 Completed Date: 1997-02-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8108529 Medline TA: Biol Cell Country: FRANCE |
Other Details:
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Languages: eng Pagination: 75-82 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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pharmacology Aminoisobutyric Acids / metabolism, pharmacokinetics* Animals Biological Transport / drug effects, physiology Cell Aging / physiology Cell Division / drug effects Enzyme Inhibitors / pharmacology Genistein Glucose / pharmacology Glutamic Acid / pharmacology Hypertonic Solutions Isoflavones / pharmacology Leishmania donovani / cytology, metabolism* Osmolar Concentration Proline / pharmacology Protein Kinase Inhibitors* Staurosporine / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R01 A126523//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Aminoisobutyric Acids; 0/Enzyme Inhibitors; 0/Hypertonic Solutions; 0/Isoflavones; 0/Protein Kinase Inhibitors; 147-85-3/Proline; 446-72-0/Genistein; 50-99-7/Glucose; 56-41-7/Alanine; 56-86-0/Glutamic Acid; 62-57-7/2-aminoisobutyric acid; 62996-74-1/Staurosporine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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