| Uptake and processing of serine: pyruvate aminotransferase precursor by rat liver mitochondria in vitro and in vivo. | |
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MedLine Citation:
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PMID: 6725236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Processing and uptake of the precursor of serine: pyruvate aminotransferase [EC 2.6.1.51] by mitochondria were studied in vitro and in vivo. Serine: pyruvate aminotransferase was synthesized mainly on free ribosomes as judged by immunoprecipitation of puromycin-labeled nascent peptides prepared from free and bound ribosomes. The precursor of rat liver serine:pyruvate aminotransferase (pSPT) synthesized in vitro was post-translationally processed to an apparently mature form by isolated rat liver mitochondria. Available evidence indicated that the processed product was localized in the matrix of mitochondria. Mature serine:pyruvate aminotransferase did not inhibit the in vitro processing, suggesting that the extra peptide was necessary for the mitochondrial uptake of the precursor. In the livers of rats fed a vitamin B6-deficient high-protein diet, the induction by glucagon of serine:pyruvate aminotransferase occurred and most of the induced enzyme existed in mitochondria as the apo-form, suggesting that pSPT was taken up by mitochondria and processed in the apo-form under the conditions employed. In the in vitro system, on the other hand, the processing of pSPT proceeded both in the absence and presence of pyridoxal 5'-phosphate. Should the precursor also bind the prosthetic molecule, therefore, it would be transported into mitochondria in both the apo- and holo-forms. When isolated rat hepatocytes were labeled with [35S]methionine, labeled pSPT appeared in the cytosolic fraction and was transported rapidly into mitochondria in association with the processing. This uptake and processing were inhibited by a fluorescent laser dye, rhodamine 123, and the precursor accumulated in the cytosol in the presence of the dye. |
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Authors:
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T Oda; A Ichiyama; S Miura; M Mori |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 95 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1984 Mar |
Date Detail:
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Created Date: 1984-07-09 Completed Date: 1984-07-09 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 815-24 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Enzyme Precursors / metabolism* Immunochemistry Male Mitochondria, Liver / enzymology* Protein Processing, Post-Translational Pyridoxine / physiology Rats Rats, Inbred Strains Subcellular Fractions / enzymology Transaminases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Precursors; 65-23-6/Pyridoxine; EC 2.6.1.-/Transaminases; EC 2.6.1.51/serine-pyruvate aminotransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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