Document Detail

Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation.
MedLine Citation:
PMID:  20358123     Owner:  NLM     Status:  MEDLINE    
Photodynamic therapy (PDT) using Photofrin and, recently, Foscan has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan) after incubation with 200 or 400 ng ml(-1) mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination.
Tobias Kiesslich; Daniel Neureiter; Beate Alinger; Gerhard L Jansky; Juergen Berlanda; Vahagn Mkrtchyan; Matthias Ocker; Kristjan Plaetzer; Frieder Berr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology     Volume:  9     ISSN:  1474-9092     ISO Abbreviation:  Photochem. Photobiol. Sci.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-06     Completed Date:  2010-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101124451     Medline TA:  Photochem Photobiol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  734-43     Citation Subset:  IM    
Department of Internal Medicine I, Paracelsus Medical University/Salzburger Landeskliniken (SALK), Muellner Hauptstrasse 48, 5020, Salzburg, Austria.
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MeSH Terms
Antineoplastic Agents* / pharmacokinetics,  toxicity
Biliary Tract Neoplasms / physiopathology*
Cell Differentiation* / drug effects
Cell Line, Tumor
Cell Proliferation
Organophosphorus Compounds / pharmacokinetics*,  toxicity*
Tumor Markers, Biological*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Organophosphorus Compounds; 0/Tumor Markers, Biological; 124-64-1/tetramethylolphosphonium chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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