Document Detail

Uptake and persistence of Mycobacterium avium subsp. paratuberculosis in human monocytes.
MedLine Citation:
PMID:  22890992     Owner:  NLM     Status:  MEDLINE    
Mycobacterium avium subsp. paratuberculosis is a bacterium sometimes found in human blood and tissue samples that may have a role in the etiology of Crohn's disease in humans. To date, however, there have been few studies examining the interactions of these bacteria with human cells. Using the THP-1 human monocytic cell line, this study shows that the uptake and trafficking of M. avium subsp. paratuberculosis in human cells are cholesterol dependent and that these bacteria localize to cholesterol-rich compartments that are slow to acidify. M. avium subsp. paratuberculosis bacteria containing phagosomes stain for the late endosomal marker Rab7, but recruitment of the Rab7-interacting lysosomal protein that regulates the fusion of bacterium-containing phagosomes with lysosomal compartments and facilitates subsequent bacterial clearance is significantly reduced. Disruption of phagosome acidification via this mechanism may contribute to M. avium subsp. paratuberculosis persistence in human cells, but there was no evidence that internalized M. avium subsp. paratuberculosis also affects the survival of bacteria taken up during a secondary phagocytic event.
Dayle A Keown; David A Collings; Jacqueline I Keenan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-13
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-11     Completed Date:  2013-03-14     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3768-75     Citation Subset:  IM    
Department of Surgery, University of Otago Christchurch, Christchurch, New Zealand.
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MeSH Terms
Cell Line
Cholesterol / analysis
Flow Cytometry
Monocytes / microbiology*
Mycobacterium avium subsp. paratuberculosis / chemistry,  metabolism*
Phagosomes / microbiology*
rab GTP-Binding Proteins / metabolism*
Reg. No./Substance:
152989-05-4/rab7 protein; 57-88-5/Cholesterol; EC 3.6.1.-/rab GTP-Binding Proteins

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