Document Detail

Uptake and metabolism of plasma-derived erucic acid by rat brain.
MedLine Citation:
PMID:  16525189     Owner:  NLM     Status:  MEDLINE    
We examined the ability of erucic acid (22:1n-9) to cross the blood-brain barrier (BBB) by infusing [14-14C]22:1n-9 (170 microCi/kg, iv and icv) into awake, male rats. [1-14C]arachidonic acid (20:4n-6) [intravenous (i.v.)] was the positive control. After i.v. infusion, 0.011% of the plasma [14-14C]22:1n-9 was extracted by the brain, compared with 0.055% of the plasma [1-14C]20:4n-6. The [14-14C]22:1n-9 was extensively beta-oxidized (60%), compared with 30% for [1-14C]20:4n-6. Although 20:4n-6 was targeted primarily to phospholipid pools, 22:1n-9 was targeted to cholesteryl esters, triglycerides, and phospholipids. When [14-14C]22:1n-9 was infused directly into the fourth ventricle of the brain [intracerebroventricular (i.c.v.)] for 7 days, 60% of the tracer entered the phospholipid pools, similar to the distribution observed for [1-14C]20:4n-6. This demonstrates plasticity in the ability of the brain to esterify 22:1n-9 in an exposure-dependent manner. In i.v. and i.c.v. infused rats, a significant amount of tracer found in the phospholipid pools underwent sequential rounds of chain shortening and was found as [12-14C]20:1n-9 and [10-14C]oleic acid. These results demonstrate for the first time that intact 22:1n-9 crosses the BBB, is incorporated into specific lipid pools, and is chain-shortened.
Mikhail Y Golovko; Eric J Murphy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-08
Journal Detail:
Title:  Journal of lipid research     Volume:  47     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-23     Completed Date:  2006-07-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1289-97     Citation Subset:  IM    
Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota, Grand Forks, ND 58202-9037, USA.
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MeSH Terms
Arachidonic Acid / metabolism,  pharmacokinetics
Brain / metabolism*
Carbon Radioisotopes / analysis
Erucic Acids / blood,  metabolism,  pharmacokinetics*
Myocardium / metabolism
Oleic Acid / metabolism
Rats, Sprague-Dawley
Grant Support
1P20 RR17699-01/RR/NCRR NIH HHS
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Erucic Acids; 1072-39-5/erucic acid; 112-80-1/Oleic Acid; 506-32-1/Arachidonic Acid

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