Document Detail


Uptake and kinetics of 5-aminolevulinic acid in oral squamous cell carcinoma.
MedLine Citation:
PMID:  20409686     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic diagnosis (PDD) is a form of cancer detection based on the administration of an exogenous photo-activated compound that accumulates in malignant cells, followed by appropriate photo irradiation. The authors describe a spectroscopic study of 5-aminolevulinic acid (5-ALA)-generated photosensitizer protoporphyrin IX (PpIX) fluorescence in human oral squamous cell carcinoma (OSCC) cell lines to validate its clinical use. 5-ALA-induced PpIX fluorescence intensity was measured in the presence and absence of deferoxamine mesylate (DFO). Two, one and two cell lines produced poorly, moderately and well differentiated carcinomas, respectively, on transplantation in scid mice. The fluorescence intensity was high in the poorly differentiated cell lines, followed by the moderately differentiated cell line; the intensity of the well differentiated cell lines was low and not significantly different from that of normal keratinocytes in the absence of DFO. It was elevated to the level of poorly differentiated cell lines following DFO treatment. This elevation was not observed in normal keratinocytes. The results indicate that DFO enhances the photodynamic sensitivity of 5-ALA in non-responsive carcinoma cells as a result of increased cellular accumulation by inhibiting haeme biosynthesis. This PDD system can be applied clinically to the detection of OSCC irrespective of the degree of differentiation.
Authors:
M Uekusa; K Omura; Y Nakajima; S Hasegawa; H Harada; K-I Morita; H Tsuda
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-20
Journal Detail:
Title:  International journal of oral and maxillofacial surgery     Volume:  39     ISSN:  1399-0020     ISO Abbreviation:  Int J Oral Maxillofac Surg     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-16     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605826     Medline TA:  Int J Oral Maxillofac Surg     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  802-5     Citation Subset:  D; IM    
Copyright Information:
Copyright 2010 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Oral and Maxillofacial Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids, Neutral / diagnostic use,  pharmacokinetics*,  radiation effects
Animals
Carcinoma, Squamous Cell / metabolism*,  pathology
Cell Differentiation
Deferoxamine / diagnostic use*
Drug Combinations
Drug Synergism
Fluorescence
Humans
Male
Mice
Mice, SCID
Mouth Neoplasms / metabolism*,  pathology
Photochemotherapy / methods
Photosensitizing Agents / diagnostic use*
Protoporphyrins / diagnostic use*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Amino Acids, Neutral; 0/Drug Combinations; 0/Photosensitizing Agents; 0/Protoporphyrins; 553-12-8/protoporphyrin IX; 660-88-8/5-aminovaleric acid; 70-51-9/Deferoxamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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