| Uptake and intracellular routing of peroxidase-conjugated immunoglobulin-G by the perfused human placenta. | |
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MedLine Citation:
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PMID: 2401009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Selected lobules of term human placenta were extracorporeally perfused and human immunoglobulin-G complexed to horseradish peroxidase (IgG-HRP) was added to the maternal perfusate. After different durations of perfusion IgG-HRP was visualised by use of diamino-benzidine cytochemistry. Within the first 10 min of perfusion IgG-HRP was found bound to microvilli and coated pits of the syncytiotrophoblast; internalisation into coated vesicles and tubulo-vesicular bodies was also observed. Subsequently, IgG-HRP was found in multivesicular bodies and by 30 min appeared in basal vesicles, the frequency of the latter event increasing with time. No routing of IgG-HRP into Golgi regions or lysosomes could be detected. by 60 min IgG-HRP was found in a few caveolae of fetal endothelium of both terminal and intermediate villi. IgG-HRP was not found in intercellular clefts of the endothelium. The pattern of uptake and routing observed suggests a receptor-mediated transcytosis of IgG-HRP across the syncytiotrophoblast and a transcellular pathway through the endothelium. |
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Authors:
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L Leach; B M Eaton; J A Firth; S F Contractor |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell and tissue research Volume: 261 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-10-22 Completed Date: 1990-10-22 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 383-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Endocytosis* Endothelium / analysis Female Fetus / metabolism Horseradish Peroxidase / pharmacokinetics Humans Immunity, Maternally-Acquired* Immunoglobulin G / pharmacokinetics* Microscopy, Electron Perfusion Placenta / cytology, metabolism* Pregnancy Trophoblasts / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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