Document Detail

Uptake and intracellular routing of peroxidase-conjugated immunoglobulin-G by the perfused human placenta.
MedLine Citation:
PMID:  2401009     Owner:  NLM     Status:  MEDLINE    
Selected lobules of term human placenta were extracorporeally perfused and human immunoglobulin-G complexed to horseradish peroxidase (IgG-HRP) was added to the maternal perfusate. After different durations of perfusion IgG-HRP was visualised by use of diamino-benzidine cytochemistry. Within the first 10 min of perfusion IgG-HRP was found bound to microvilli and coated pits of the syncytiotrophoblast; internalisation into coated vesicles and tubulo-vesicular bodies was also observed. Subsequently, IgG-HRP was found in multivesicular bodies and by 30 min appeared in basal vesicles, the frequency of the latter event increasing with time. No routing of IgG-HRP into Golgi regions or lysosomes could be detected. by 60 min IgG-HRP was found in a few caveolae of fetal endothelium of both terminal and intermediate villi. IgG-HRP was not found in intercellular clefts of the endothelium. The pattern of uptake and routing observed suggests a receptor-mediated transcytosis of IgG-HRP across the syncytiotrophoblast and a transcellular pathway through the endothelium.
L Leach; B M Eaton; J A Firth; S F Contractor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  261     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-10-22     Completed Date:  1990-10-22     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  383-8     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, United Kingdom.
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MeSH Terms
Endothelium / analysis
Fetus / metabolism
Horseradish Peroxidase / pharmacokinetics
Immunity, Maternally-Acquired*
Immunoglobulin G / pharmacokinetics*
Microscopy, Electron
Placenta / cytology,  metabolism*
Trophoblasts / metabolism
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Immunoglobulin G; EC 1.11.1.-/Horseradish Peroxidase

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