| Uptake of glutamate and cysteine in C-6 glioma cells and in cultured astrocytes. | |
| | |
MedLine Citation:
|
PMID: 1977889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The uptake of glutamate in rat glioma C-6 cells and cultured astrocytes derived from rat cerebral hemispheres was found to be mediated by a Na(+)-dependent and a Na(+)-independent system. The Na(+)-dependent system was inhibited by aspartate and was consistent with the commonly occurring system designated system X-AG. The Na(+)-independent system was inhibited by cystine and was consistent with system x-c described in various types of cells in the periphery. It was also found that quisqualate selectively and competitively interfered with the Na(+)-independent glutamate uptake. In C-6 cells, the glutamate uptake via systems X-AG and x-c accounted for approximately 35% and 55% of the total uptake, respectively, at 0.05 mM glutamate. In cultured astrocytes, the glutamate uptake via system X-AG was very potent, whereas the uptake via system xc- was relatively weak and its contribution to the total uptake of glutamate seemed almost negligible. However, in both C-6 cells and astrocytes, system xc- was necessary for the uptake of cystine, another substrate of system xc-. Cystine in the culture medium was an essential precursor of glutathione, and the inhibition of the cystine uptake by excess glutamate as a competitor led to a severe deficiency in glutathione, followed by cell degeneration. |
| | |
Authors:
|
Y Cho; S Bannai |
Related Documents
:
|
14763819 - L-glutamate enhances the expression of thermus maltogenic amylase in escherichia coli. 683409 - Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures. 13759 - Ammonium uptake and metabolism by mitrogen fixing bacteria. ii. klebsiella pneumoniae. 1982459 - Effect of 8-bromo-camp and dexamethasone on glutamate metabolism in rat astrocytes. 19707849 - Effects of epigallocatechin gallate on rotenone-injured murine brain cultures. 10988329 - Flupirtine and retigabine prevent l-glutamate toxicity in rat pheochromocytoma pc 12 ce... 10207189 - Design of biomimetic habitats for tissue engineering with p-15, a synthetic peptide ana... 8171439 - Cocaine toxicity in cultured chicken hepatocytes: role of cytochrome p450. 17502159 - Chondrogenic potential of human synovial mesenchymal stem cells in alginate. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 55 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1990 Dec |
Date Detail:
|
Created Date: 1990-12-20 Completed Date: 1990-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2091-7 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Tsukuba University Medical School, Ibaraki, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
pharmacology Animals Astrocytes / metabolism* Biological Transport Cells, Cultured Cystine / pharmacokinetics* Glioma / metabolism*, pathology Glutamates / antagonists & inhibitors, pharmacokinetics* Glutamic Acid Glutathione / metabolism Kinetics Sodium / pharmacology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Glutamates; 56-86-0/Glutamic Acid; 56-89-3/Cystine; 70-18-8/Glutathione; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparison of the in vitro receptor binding properties of N-[3H]methylspiperone and [3H]raclopride t...
Next Document: Homologous desensitization of the D1 dopamine receptor.