Document Detail

Uptake of fatty acids by Mycoplasma capricolum.
MedLine Citation:
PMID:  2834319     Owner:  NLM     Status:  MEDLINE    
The energy requirements for fatty acid uptake by Mycoplasma capricolum were studied. Fatty acid transport and esterification to phospholipid appeared to be tightly coupled, since there was little intracellular accumulation of free fatty acid. Uptake was blocked by iodoacetate, n-ethylmaleimide, and p-chloromercuribenzoate. Glucose, glycerol, and potassium ions were necessary for fatty acid uptake by whole cells. A reduction in uptake was observed in cells treated with valinomycin or dicyclohexylcarbodiimide. The effect of temperature on the rate of oleate uptake showed a discontinuity at 24 degrees C. Above 24 degrees C an energy of activation of 4.6 kcal (ca. 19.2 kJ)/mol was obtained. The data suggest that uptake of fatty acid by M. capricolum is an energy-linked, protein-mediated process. A membrane-bound enzyme activity that catalyzed the synthesis of fatty acyl-hydroxamate was demonstrated. This activity was virtually independent or only marginally dependent on coenzyme A, depending on the assay system, but was stimulated approximately twofold by ATP.
J Dahl
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-09     Completed Date:  1988-06-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2022-6     Citation Subset:  IM    
Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138.
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MeSH Terms
Biological Transport
Dicyclohexylcarbodiimide / pharmacology
Fatty Acids / metabolism*
Glucose / metabolism
Glycerol / metabolism
Hydrogen-Ion Concentration
Mycoplasma / drug effects,  enzymology,  metabolism*
Nucleotidyltransferases / metabolism
Phosphatidylglycerols / biosynthesis
Phospholipids / biosynthesis
Phosphotransferases / metabolism
Potassium / pharmacology
Sulfhydryl Reagents / pharmacology
Transferases (Other Substituted Phosphate Groups)*
Valinomycin / pharmacology
Reg. No./Substance:
0/Esters; 0/Fatty Acids; 0/Phosphatidylglycerols; 0/Phospholipids; 0/Sulfhydryl Reagents; 2001-95-8/Valinomycin; 50-99-7/Glucose; 538-75-0/Dicyclohexylcarbodiimide; 56-81-5/Glycerol; 7440-09-7/Potassium; EC 2.7.-/Phosphotransferases; EC 2.7.7.-/Nucleotidyltransferases; EC cytidylyltransferase; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 3-phosphatidyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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