Document Detail


Uptake of doxorubicin by cultured kidney epithelial cells LLC-PK1.
MedLine Citation:
PMID:  9635513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The renal handling of doxorubicin (DXR) was investigated using a kidney epithelial cell line, LLC-PK1. The uptake of DXR by LLC-PK1 cells cultured on plastic dishes was shown to be temperature and concentration dependent. The initial uptake of DXR was slightly saturable. The Km and Vmax of the saturable component were calculated to be 20.2 microM, and 0.355 nmol/mg protein/10 min, respectively. The release of DXR from LLC-PK1 cells was very slow at 37 degrees C and almost negligible at 4 degrees C, indicating that most of the DXR in the cells irreversibly binds to cellular constituents and that only a slight amount of unbound DXR participates in the efflux out of the cells. DXR uptake at 37 degrees C was significantly decreased in the presence of 2,4-dinitrophenol. However, organic cations and aminoglycoside antibiotics, such as tetraethylammonium, N1-methylnicotinamide, guanidine, gentamicin and neomycin, did not inhibit DXR uptake, suggesting that a process distinct from the organic cation transport system and absorptive endocytosis might be involved in the uptake of DXR by LLC-PK1 cells.
Authors:
M Sasaya; I Wada; M Shida; M Sato; Y Hatakeyama; H Saitoh; M Takada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  21     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-24     Completed Date:  1998-08-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  527-9     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Antibiotics, Antineoplastic / metabolism*
Antimetabolites / pharmacology
Cell Count
Chromatography, High Pressure Liquid
Doxorubicin / metabolism*
Endocytosis / drug effects
Kidney / drug effects,  metabolism*
Kinetics
LLC-PK1 Cells
Rats
Swine
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Antimetabolites; 23214-92-8/Doxorubicin; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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