| Uptake of dodecanedioic acid by isolated rat liver. | |
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MedLine Citation:
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PMID: 9074817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The uptake of dodecanedioic acid (C12); a dicarboxylic acid with 12 carbon atoms, was studied in the isolated perfused rat liver. Fifty mumol of C12 were injected as a bolus into the perfusing liver solution. The concentration of C12 in perfusate samples taken over 2 h from the beginning of the experiments were analyzed by high performance liquid chromatography. An in vitro experimental session was performed to determine the binding curve of C12 to defatted bovine serum albumin. These data were then used to compute the perfusate C12 free fraction. The number of binding sites on the albumin molecule was equal to 4.29 +/- 0.21 (S.E.), while the affinity constant was 6.33 +/- 0.87 x 10(3). M-1. Experimental values of perfusate C12 concentration versus time were individually plotted and fitted to a monoexponential decay for each liver perfused. The predicted C12 concentration at time zero averaged 0.354 +/- 0.0375 mumol/ml. Prom this value the apparent volume of distribution of C12 was obtained and corresponded to 153.02 +/- 14.56 ml. The disappearance rate constant from the perfusate was 0.0278 +/- 0.0030 min-1. The C12 half life was 26.6 +/- 2.3 min. The mean hepatic clearance from the perfusate was 4.08 +/- 0.38 ml/min. In conclusion, C12 is quickly taken up by the liver so that in about 100 min it was completely cleared from the perfusate. |
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Authors:
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A V Greco; G Mingrone; A De Gaetano; L Amigo; L Puglielli; M Castagneto; F Nervi |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 258 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-05-22 Completed Date: 1997-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 209-18 Citation Subset: IM |
Affiliation:
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Instituto di Medicina Interna e Geriatria, Largo A. Gemelli, Rome, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Dicarboxylic Acids / pharmacokinetics* Half-Life Liver / metabolism* Male Perfusion Rats Rats, Wistar Serum Albumin, Bovine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Dicarboxylic Acids; 0/Serum Albumin, Bovine; 693-23-2/dodecanedioic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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