Document Detail


Uptake, distribution, and oxidation of fatty acids by Leishmania mexicana amastigotes.
MedLine Citation:
PMID:  3037056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acid uptake, distribution, and beta-oxidation were investigated in Leishmania mexicana amastigotes. The uptake of radiolabeled palmitic, stearic, and oleic acids was similar, reaching 3-6 nmol/10(8) cells in 2 min and 8-12 nmol/10(8) cells in 60 min. The percent of radiolabeled fatty acid that was esterified in the form of triglycerides or phospholipids increased from less than 25% at 2 min to 65-86% at 60 min. The dehydrogenase(s) in an amastigote granule fraction were unusual in that the Vmax for long-chain substrates (0.95-1.6 delta Abs units/min-mg protein) approximated the Vmax for short-chain substrates (0.82-2.0 delta Abs units/min-mg protein), and the Km for long-chain substrates was high (approximately 250 microM), in contrast to data for a mammalian liver mitochondrial fraction. The high Vmax and Km for long-chain substrates suggest a biochemical mechanism for the postulated high utilization of fatty acids as an energy source for amastigotes. Although the primary anti-leishmanial agent, Sb in the form of Pentostam, inhibited oxidation of palmitic acid to CO2 by intact organisms, Sb did not significantly inhibit fatty acid uptake or esterification by organisms, or beta-oxidation by the granule fraction, and the mechanism of action of Sb remains unclear.
Authors:
J D Berman; J V Gallalee; J M Best; T Hill
Related Documents :
10964926 - Identification of the putrescine recognition site on polyamine transport protein pote.
1130516 - Liver uptake of amino acids and carbohydrates during a single circulatory passage.
20492996 - Characterization of the uptake of gaba, nipecotic acid and cis-4-oh-nipecotic acid in c...
7102556 - Myocardial metabolic alterations after contrast angiography.
2515936 - Distribution of omega-3 and omega-6 fatty acids in the ether- and ester-linked phosphog...
4091866 - N-alkyl barbiturates. a series of compounds for the study of metabolic structure-activi...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of parasitology     Volume:  73     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-26     Completed Date:  1987-08-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-60     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acyl-CoA Dehydrogenase
Antimony Sodium Gluconate / pharmacology
Fatty Acid Desaturases / analysis
Fatty Acids / metabolism*
Kinetics
Leishmania mexicana / metabolism*
Oxidation-Reduction
Palmitic Acid
Palmitic Acids / metabolism
Chemical
Reg. No./Substance:
0/Antimony Sodium Gluconate; 0/Fatty Acids; 0/Palmitic Acids; 57-10-3/Palmitic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.3.99.3/Acyl-CoA Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Congenital mesoblastic nephroma: a clinicoradiologic study of 17 cases representing the pathologic s...
Next Document:  Itraconazole treatment of experimental systemic candidiasis in male rats.