| Uptake, distribution, and oxidation of fatty acids by Leishmania mexicana amastigotes. | |
| | |
MedLine Citation:
|
PMID: 3037056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fatty acid uptake, distribution, and beta-oxidation were investigated in Leishmania mexicana amastigotes. The uptake of radiolabeled palmitic, stearic, and oleic acids was similar, reaching 3-6 nmol/10(8) cells in 2 min and 8-12 nmol/10(8) cells in 60 min. The percent of radiolabeled fatty acid that was esterified in the form of triglycerides or phospholipids increased from less than 25% at 2 min to 65-86% at 60 min. The dehydrogenase(s) in an amastigote granule fraction were unusual in that the Vmax for long-chain substrates (0.95-1.6 delta Abs units/min-mg protein) approximated the Vmax for short-chain substrates (0.82-2.0 delta Abs units/min-mg protein), and the Km for long-chain substrates was high (approximately 250 microM), in contrast to data for a mammalian liver mitochondrial fraction. The high Vmax and Km for long-chain substrates suggest a biochemical mechanism for the postulated high utilization of fatty acids as an energy source for amastigotes. Although the primary anti-leishmanial agent, Sb in the form of Pentostam, inhibited oxidation of palmitic acid to CO2 by intact organisms, Sb did not significantly inhibit fatty acid uptake or esterification by organisms, or beta-oxidation by the granule fraction, and the mechanism of action of Sb remains unclear. |
| | |
Authors:
|
J D Berman; J V Gallalee; J M Best; T Hill |
Related Documents
:
|
10964926 - Identification of the putrescine recognition site on polyamine transport protein pote. 1130516 - Liver uptake of amino acids and carbohydrates during a single circulatory passage. 20492996 - Characterization of the uptake of gaba, nipecotic acid and cis-4-oh-nipecotic acid in c... 7102556 - Myocardial metabolic alterations after contrast angiography. 2515936 - Distribution of omega-3 and omega-6 fatty acids in the ether- and ester-linked phosphog... 4091866 - N-alkyl barbiturates. a series of compounds for the study of metabolic structure-activi... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of parasitology Volume: 73 ISSN: 0022-3395 ISO Abbreviation: J. Parasitol. Publication Date: 1987 Jun |
Date Detail:
|
Created Date: 1987-08-26 Completed Date: 1987-08-26 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7803124 Medline TA: J Parasitol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 555-60 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acyl-CoA Dehydrogenase Antimony Sodium Gluconate / pharmacology Fatty Acid Desaturases / analysis Fatty Acids / metabolism* Kinetics Leishmania mexicana / metabolism* Oxidation-Reduction Palmitic Acid Palmitic Acids / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antimony Sodium Gluconate; 0/Fatty Acids; 0/Palmitic Acids; 57-10-3/Palmitic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.3.99.3/Acyl-CoA Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Congenital mesoblastic nephroma: a clinicoradiologic study of 17 cases representing the pathologic s...
Next Document: Itraconazole treatment of experimental systemic candidiasis in male rats.