Document Detail

Uptake, distribution, and oxidation of fatty acids by Leishmania mexicana amastigotes.
MedLine Citation:
PMID:  3037056     Owner:  NLM     Status:  MEDLINE    
Fatty acid uptake, distribution, and beta-oxidation were investigated in Leishmania mexicana amastigotes. The uptake of radiolabeled palmitic, stearic, and oleic acids was similar, reaching 3-6 nmol/10(8) cells in 2 min and 8-12 nmol/10(8) cells in 60 min. The percent of radiolabeled fatty acid that was esterified in the form of triglycerides or phospholipids increased from less than 25% at 2 min to 65-86% at 60 min. The dehydrogenase(s) in an amastigote granule fraction were unusual in that the Vmax for long-chain substrates (0.95-1.6 delta Abs units/min-mg protein) approximated the Vmax for short-chain substrates (0.82-2.0 delta Abs units/min-mg protein), and the Km for long-chain substrates was high (approximately 250 microM), in contrast to data for a mammalian liver mitochondrial fraction. The high Vmax and Km for long-chain substrates suggest a biochemical mechanism for the postulated high utilization of fatty acids as an energy source for amastigotes. Although the primary anti-leishmanial agent, Sb in the form of Pentostam, inhibited oxidation of palmitic acid to CO2 by intact organisms, Sb did not significantly inhibit fatty acid uptake or esterification by organisms, or beta-oxidation by the granule fraction, and the mechanism of action of Sb remains unclear.
J D Berman; J V Gallalee; J M Best; T Hill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of parasitology     Volume:  73     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-08-26     Completed Date:  1987-08-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-60     Citation Subset:  IM    
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MeSH Terms
Acyl-CoA Dehydrogenase
Antimony Sodium Gluconate / pharmacology
Fatty Acid Desaturases / analysis
Fatty Acids / metabolism*
Leishmania mexicana / metabolism*
Palmitic Acid
Palmitic Acids / metabolism
Reg. No./Substance:
0/Antimony Sodium Gluconate; 0/Fatty Acids; 0/Palmitic Acids; 57-10-3/Palmitic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC Dehydrogenase

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