| Uptake and distribution of exogenous CoQ in the mitochondrial fraction of perfused rat liver. | |
| | |
MedLine Citation:
|
PMID: 7752844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The system of perfusing rat livers has been used to evaluate the uptake and incorporation of liposomal CoQ10 into mitochondria. After 90 minutes of perfusion the cells are strongly enriched in CoQ10 up to levels of the same order of magnitude as CoQ9. Heavy and light mitochondrial crude subcellular fractions, low in CoQ10 in control livers, contain high amounts of the quinone after perfusion; yet the purification of these fractions on a metrizamide gradient reveals that the exogenous quinone is mainly associated with the light mitochondrial subfraction, enriched in lysosomes. An increase of the NAD-dependent glutamate-malate oxidase activity is observed in CoQ10 perfused animals. As the total levels of CoQ9 + CoQ10 in these animals are not significantly modified by the CoQ10 incorporated, the observed higher activity is not ascribable to an integration of exogenous quinone into the ubiquinone pool. An antioxidant effect of extramitochondrial CoQ10 on mitochondrial functions is suggested. |
| | |
Authors:
|
M L Genova; C Bovina; G Formiggini; V Ottani; S Sassi; M Marchetti |
Related Documents
:
|
16742514 - The rate of gluconeogenesis from various precursors in the perfused rat liver. 3166024 - Net prostaglandin release by perfused rat liver after stimulation with phorbol 12-myris... 2346034 - Metabolism of the glutathione conjugate of propachlor by in situ perfused kidneys and l... 651014 - Effects of intracerebroventricular prostaglandins e2 and f2alpha on the urine outflow, ... 6844434 - Variety in the flavor of food enhances eating in the rat: a controlled demonstration. 3191474 - Effect of nutritional zinc-deficiency on o6-alkylguanine-dna-methyl-transferase activit... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Molecular aspects of medicine Volume: 15 Suppl ISSN: 0098-2997 ISO Abbreviation: Mol. Aspects Med. Publication Date: 1994 |
Date Detail:
|
Created Date: 1995-06-21 Completed Date: 1995-06-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7603128 Medline TA: Mol Aspects Med Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: s47-55 Citation Subset: IM |
Affiliation:
|
Dipartmento di Biochimica G. Moruzzi, Università di Bologna, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Fractionation Liposomes Male Metrizamide Mitochondria, Liver / metabolism* Perfusion Phosphatidylcholines Phospholipids Rats Rats, Wistar Subcellular Fractions / chemistry Ubiquinone / administration & dosage, pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
|
0/Liposomes; 0/Phosphatidylcholines; 0/Phospholipids; 1339-63-5/Ubiquinone; 303-97-9/ubiquinone 9; 31112-62-6/Metrizamide; 69279-91-0/asolectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of coenzyme Q biosynthesis.
Next Document: Interactions between ubiquinones and vitamins in membranes and cells.