Document Detail

Uptake of dihomo-gamma-linolenic acid by murine macrophages increases series-1 prostaglandin release following lipopolysaccharide treatment.
MedLine Citation:
PMID:  20347284     Owner:  NLM     Status:  MEDLINE    
Administration of dihomo-gamma-linolenic acid is useful for atopic dermatitis and atherosclerosis in mice; however, the metabolites of dihomo-gamma-linolenic acid have been little studied. We employed a method which enabled simultaneous analysis of nine prostaglandins using liquid chromatography-tandem mass spectrometry, and determined the concentrations of prostaglandins in the supernatants of cultures of mouse peritoneal macrophages stimulated with lipopolysaccharide after pre-incubation with dihomo-gamma-linolenic acid, arachidonic acid, or eicosapentaenoic acid. Accumulated prostaglandin concentrations from mouse macrophages with dihomo-gamma-linolenic acid uptake increased in a dihomo-gamma-linolenic acid concentration-dependent fashion. These increases were mainly due to prostaglandin D(1) and prostaglandin E(1). The order of accumulated prostaglandin concentrations was dihomo-gamma-linolenic acid>arachidonic acid>eicosapentaenoic acid in supernatants with the same concentration of polyunsaturated fatty acid. Since mouse macrophages can clearly produce series-1 prostaglandins, they must be formed in vivo. These findings suggest that the effects of dihomo-gamma-linolenic acid on diseases may be due to series-1 prostaglandins.
Saki Kakutani; Hiroshi Kawashima; Takao Tanaka; Akiko Shiraishi-Tateishi; Yoshinobu Kiso
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Publication Detail:
Type:  Journal Article     Date:  2010-03-26
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  83     ISSN:  1532-2823     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  23-9     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Institute for Health Care Science, Suntory Wellness Ltd., Shimamoto, Osaka 618-8503, Japan.
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MeSH Terms
8,11,14-Eicosatrienoic Acid / pharmacology*
Alprostadil / secretion*
Arachidonic Acid / pharmacology
Dermatitis, Atopic / drug therapy,  metabolism
Dose-Response Relationship, Drug
Eicosanoic Acids / pharmacology
Lipopolysaccharides / pharmacology*
Macrophages, Peritoneal / cytology,  secretion*
Mice, Inbred BALB C
Prostaglandins D / secretion*
Reg. No./Substance:
0/Eicosanoic Acids; 0/Lipopolysaccharides; 0/Prostaglandins D; 506-32-1/Arachidonic Acid; 7324-41-6/8,11,14-Eicosatrienoic Acid; 745-65-3/Alprostadil

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