| Uptake and cellular localization of exogenous lipids by Giardia lamblia, a primitive eukaryote. | |
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MedLine Citation:
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PMID: 9207743 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Giardia lamblia trophozoites are unable to carry out de novo lipid synthesis. It is therefore likely that lipids are acquired from the small intestine of the host, in which the trophozoites are exposed to free and conjugated fatty acids, various sterols, phospholipids, bile acids, and bile-lipid mixed micelles. Here we show that G. lamblia is capable of taking up exogenous phosphatidylcholine (PC), phosphatidylinositol (PI), sphingomyelin (SM), cholesterol, ceramide (Cer), and fatty acids. Results from epifluorescence and high-resolution confocal microscopy suggest that fluorescent analogs of SM and PC were accumulated in the plasma membranes, whereas palmitic acid and Cer were localized intracellularly. Interestingly, many of these analogs were also concentrated in perinuclear regions. Similar labeling patterns were observed when the fluorescent analogs were delivered to the parasite via liposomes. To test whether G. lamblia was capable of esterifying exogenous fatty acids into membrane or cellular phospholipids, trophozoites were pulse-labeled with 3H-labeled palmitic or myristic acids and the phospholipids analyzed by thin-layer chromatography. Results document that G. lamblia was able to incorporate exogenous fatty acids into various phospholipids, i.e., PI, PC, PE, and PG. Interestingly, a major portion of radiolabeled fatty acids was incorporated into PG, a phospholipid characteristic of prokaryotic membranes. |
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Authors:
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T L Stevens; G R Gibson; R Adam; J Maier; M Allison-Ennis; S Das |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental parasitology Volume: 86 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1997 Jun |
Date Detail:
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Created Date: 1997-07-14 Completed Date: 1997-07-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 133-43 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Texas, El Paso 79968-0519, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Esterification Eukaryotic Cells / metabolism* Fatty Acids / metabolism Fluorescent Dyes Giardia / metabolism* Image Processing, Computer-Assisted Lipid Metabolism* Microscopy, Confocal Microscopy, Fluorescence |
| Grant Support | |
ID/Acronym/Agency:
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AI 136597/AI/NIAID NIH HHS; GM 08012/GM/NIGMS NIH HHS; RR 08124/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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