Document Detail


Uptake of antigen encapsulated in polyethylcyanoacrylate nanoparticles by D1-dendritic cells.
MedLine Citation:
PMID:  15025183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyethylcyanoacrylate (PECA) nanoparticles were prepared by interfacial polymerization of a water-in-oil microemulsion. Nanoparticles were isolated from the polymerization template by sequential ethanol washing and centrifugation. A nanocapsule preparation yielding the original particle size and distribution following redispersion in an aqueous solution was achieved by freeze-drying the isolated nanoparticles in a solution of 5% w/v sugar. The cytotoxicity and uptake of nanocapsules by dendritic cells was investigated using a murine-derived cell line (D1). PECA nanoparticles were found to adversely effect cell viability at concentrations greater than 10 microg/ml of polymer in the culture medium. In comparison to antigen in solution, cell uptake of antigen encapsulated within nanoparticles was significantly higher at both 4 and 37 degrees C. Following a 24 h incubation period, the percentage of cells taking-up antigen was also increased when antigen was encapsulated in nanoparticles as compared to antigen in solution. The uptake of nanoparticles and the effect of antigen formulation on morphological cell changes indicative of cell maturation were also investigated by scanning electron microscopy (SEM). SEM clearly demonstrated the adherence of nanoparticles to the cell surface. Incubation of D1 dendritic cells with nanoparticles containing antigen also resulted in morphological changes indicative of cell maturation similar to that observed when the cells were incubated with lipopolysaccharide. In contrast, cells incubated with antigen solution did not demonstrate such morphological changes and appeared similar to immature cells that had not been exposed to antigen.
Authors:
T Pitaksuteepong; N M Davies; M Baird; T Rades
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Die Pharmazie     Volume:  59     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-17     Completed Date:  2004-04-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  134-42     Citation Subset:  IM    
Affiliation:
Colloidal Drug Delivery Group, New Zealand National School of Pharmacy, Dunedin, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / administration & dosage*,  metabolism
Cell Survival / drug effects
Cells, Cultured
Chemistry, Physical
Cyanoacrylates / chemistry*
Dendritic Cells / metabolism*
Emulsions
Flow Cytometry
Freeze Drying
Mice
Microscopy, Electron, Scanning
Microspheres
Ovalbumin / immunology,  metabolism
Particle Size
Physicochemical Phenomena
Chemical
Reg. No./Substance:
0/Antigens; 0/Cyanoacrylates; 0/Emulsions; 25067-30-5/cyacrin; 9006-59-1/Ovalbumin

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