Document Detail

Uptake, accumulation, and egress of erythromycin by tissue culture cells of human origin.
MedLine Citation:
PMID:  3994346     Owner:  NLM     Status:  MEDLINE    
The ability of erythromycin A base to penetrate and accumulate in tissue culture cells of human origin was investigated. The antibiotic was highly concentrated by early passage cells of normal bronchus, kidney, liver, lung, and skin and by cancer cells derived from breast, liver, and lung. Intracellular levels 4 to 12 times that of the extracellular milieu were obtained in both early-passage and transformed cells. The total quantity of erythromycin accumulated depended on the extracellular concentration of antibiotic, but the cellular/extracellular ratios were, for the most part, independent of the initial extracellular drug concentration. In all cell types tested, the accumulated antibiotic rapidly egressed when cells were incubated in antibiotic-free medium. Bioactivity assays demonstrated that the expelled drug was unmetabolized, fully active antibiotic. The concentration of erythromycin by a variety of human cell types probably accounts, in part, for the effectiveness of the antibiotic against intracellular parasites such as Legionella and Chlamydia spp.
J R Martin; P Johnson; M F Miller
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  27     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-06-10     Completed Date:  1985-06-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-9     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Centrifugation, Density Gradient
Culture Techniques
Erythromycin / metabolism*,  pharmacology
Neoplasms / metabolism
Tissue Distribution
Reg. No./Substance:

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