| Uptake of 13-hydroperoxylinoleic acid by cultured cells. | |
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MedLine Citation:
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PMID: 10195919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidized free fatty acids have profound effects on cultured cells. However, little is known about whether these effects depend on their uptake and metabolism by cells or primarily involve their interaction with cell-surface components. We determined the uptake and metabolism of unoxidized (linoleic or oleic acid) and oxidized linoleic acid (13-hydroperoxyoctadecadienoic acid, 13-HPODE) by endothelial cells, smooth muscle cells, and macrophages. We show that 13-HPODE is poorly taken up by cells. The levels of uptake were dependent on the cell type but were independent of the expression of CD36. 13-HPODE was also poorly used by microsomal lysophosphatidylcholine acyltransferase that is involved in the formation of phosphatidylcholine. Based on these results, we suggest that most of the biological effects of 13-HPODE and other oxidized free fatty acids on cells might involve a direct interaction with cell-surface components. Alternatively, very small amounts of oxidized free fatty acids that enter the cell may have effects, analogous to those of hormones or prostanoids. |
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Authors:
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N Augé; N Santanam; N Mori; C Keshava; S Parthasarathy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 19 ISSN: 1079-5642 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-05-17 Completed Date: 1999-05-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 925-31 Citation Subset: IM |
Affiliation:
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Department of Gynecology and Obstetrics, Emory University, Atlanta, GA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD36 / biosynthesis Binding, Competitive Carbon Radioisotopes Cell Line Cells, Cultured / metabolism* Cholesterol Esters / metabolism Fatty Acids, Nonesterified / metabolism Humans Linoleic Acid / metabolism Linoleic Acids / metabolism* Macrophages, Peritoneal / metabolism Mice Microsomes, Liver / enzymology Oleic Acid / metabolism Rabbits Rats Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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HL 52628-01A3/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Carbon Radioisotopes; 0/Cholesterol Esters; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 34444-18-3/13-hydroperoxylinoleic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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