Document Detail


Uptake of 13-hydroperoxylinoleic acid by cultured cells.
MedLine Citation:
PMID:  10195919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidized free fatty acids have profound effects on cultured cells. However, little is known about whether these effects depend on their uptake and metabolism by cells or primarily involve their interaction with cell-surface components. We determined the uptake and metabolism of unoxidized (linoleic or oleic acid) and oxidized linoleic acid (13-hydroperoxyoctadecadienoic acid, 13-HPODE) by endothelial cells, smooth muscle cells, and macrophages. We show that 13-HPODE is poorly taken up by cells. The levels of uptake were dependent on the cell type but were independent of the expression of CD36. 13-HPODE was also poorly used by microsomal lysophosphatidylcholine acyltransferase that is involved in the formation of phosphatidylcholine. Based on these results, we suggest that most of the biological effects of 13-HPODE and other oxidized free fatty acids on cells might involve a direct interaction with cell-surface components. Alternatively, very small amounts of oxidized free fatty acids that enter the cell may have effects, analogous to those of hormones or prostanoids.
Authors:
N Augé; N Santanam; N Mori; C Keshava; S Parthasarathy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  19     ISSN:  1079-5642     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-17     Completed Date:  1999-05-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  925-31     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, Emory University, Atlanta, GA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36 / biosynthesis
Binding, Competitive
Carbon Radioisotopes
Cell Line
Cells, Cultured / metabolism*
Cholesterol Esters / metabolism
Fatty Acids, Nonesterified / metabolism
Humans
Linoleic Acid / metabolism
Linoleic Acids / metabolism*
Macrophages, Peritoneal / metabolism
Mice
Microsomes, Liver / enzymology
Oleic Acid / metabolism
Rabbits
Rats
Substrate Specificity
Grant Support
ID/Acronym/Agency:
HL 52628-01A3/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Carbon Radioisotopes; 0/Cholesterol Esters; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 34444-18-3/13-hydroperoxylinoleic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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