| Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells. | |
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MedLine Citation:
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PMID: 17765897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs. |
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Authors:
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Zhongcai Ma; BongSook Jhun; Chad K Oh |
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Publication Detail:
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Type: Journal Article Date: 2007-08-27 |
Journal Detail:
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Title: FEBS letters Volume: 581 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-09-11 Completed Date: 2008-01-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4485-90 Citation Subset: IM |
Affiliation:
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University of California, Los Angeles, UCLA School of Medicine, Division of Allergy and Immunology, Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, CA 90502, United States. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line E-Box Elements / genetics* Electrophoretic Mobility Shift Assay Humans Ionomycin / pharmacology Luciferases / genetics, metabolism Mast Cells / cytology, drug effects, metabolism* Mutagenesis, Site-Directed Phosphorylation Plasminogen Activator Inhibitor 1 / genetics*, metabolism Promoter Regions, Genetic / genetics Protein Binding Recombinant Fusion Proteins / genetics, metabolism Transcription, Genetic / drug effects Transfection Upstream Stimulatory Factors / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Activator Inhibitor 1; 0/Recombinant Fusion Proteins; 0/Upstream Stimulatory Factors; 56092-81-0/Ionomycin; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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