Document Detail


Upstream sequences modulate in vitro transcription from Drosophila yolk protein genes I and II.
MedLine Citation:
PMID:  3089778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A Drosophila transcription system was employed to study transcription in vitro from templates carrying yolk polypeptide I and II (YPI, YPII) promoter regions. The transcription start sites of YPI and YPII genes are linked by 1225 base pairs of intergenic DNA and the genes are transcribed in divergent directions. Transcription was studied in vitro from templates carrying YPI or YPII promoter regions separately. We have successively deleted intergenic DNA upstream of the YPI or the YPII transcription start site and have assayed the dependence of transcription efficiency an template concentration. The results indicate that in vitro YPI and YPII transcript synthesis is controlled by separate cis-acting DNA sequence elements, which are located between 86 and 159 base pairs upstream of the YPI transcription start site and between 161 and 341 base pairs upstream of the YPII transcription start site. These elements stimulate in vitro transcription up to fivefold. Transcription was also studied with templates which contained both YP promoters. The transcription data indicate that the intergenic DNA contains additional DNA motif(s), which apparently modulate in vitro transcription coordinately from both YP promoters in an orientation-dependent manner.
Authors:
D Voss; O Pongs
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  158     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  25-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA / analysis
Drosophila melanogaster
Egg Proteins / genetics*
Promoter Regions, Genetic
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Egg Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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