| Upstream open reading frames regulate the cell cycle-dependent expression of the RNA helicase Rok1 in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 20969870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in the uORFs resulted in increased Rok1p levels. Rok1 protein levels oscillated during the cell cycle, declining at G1/S and increasing at G2. The uAAG1 mutation caused a constitutive level of Rok1 proteins throughout the cell cycle, resulting in significant delays in mitotic bud emergence and recovery from pheromone arrest. Our study reveals that the Rok1 protein level is regulated by uORFs, which is critical in cell cycle progression. |
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Authors:
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Soonmee Jeon; Jinmi Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-20 |
Journal Detail:
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Title: FEBS letters Volume: 584 ISSN: 1873-3468 ISO Abbreviation: FEBS Lett. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-15 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4593-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Microbiology and Molecular Biology, College of Biosciences and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5' Untranslated Regions
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genetics Base Sequence Cell Cycle / genetics* DEAD-box RNA Helicases / genetics* G1 Phase / genetics Gene Expression Regulation, Fungal / genetics* Molecular Sequence Data Mutation Open Reading Frames / genetics* Protein Biosynthesis / genetics S Phase / genetics Saccharomyces cerevisiae / cytology*, enzymology, genetics* Saccharomyces cerevisiae Proteins / genetics* |
| Chemical | |
Reg. No./Substance:
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0/5' Untranslated Regions; 0/Saccharomyces cerevisiae Proteins; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/ROK1 protein, S cerevisiae |
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