| Upregulation of redox-regulating protein, thioredoxin, in endomyocardial biopsy samples of patients with myocarditis and cardiomyopathies. | |
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MedLine Citation:
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PMID: 12870673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An important role of redox regulation in myocardial diseases and heart failure has been postulated. Thioredoxin (TRX) is a redox-regulating protein. Recent studies indicated a possible association between plasma TRX concentrations and the severity of heart failure. Accordingly, we investigated the myocardial expression of TRX in patients with myocarditis and cardiomyopathies. Four cases of hypertrophic cardiomyopathy (HCM), 10 of dilated cardiomyopathy (DCM), 6 of myocarditis, and 5 of controls were studied. Right and left ventricular endomyocardial biopsy samples were obtained at the diagnostic cardiac catheterization. The samples were processed for immunohistological staining for TRX, which was done by the indirect immunoperoxidase technique. 8-hydoxy-2'-deoxyguanosine (8-OHdG), one of the major DNA base-modified products, was also detected for an established marker for oxidative stress. TRX immunoreactivity was none or trivial in control specimens. Positive TRX staining was found in 6 cases; 3 in active myocarditis and 3 in DCM. The positive staining was found in infiltrating cells and damaged myocytes in the perinecrotic lesions. Damaged myocytes were also positive for 8-OHdG All the 3 cases of DCM positive for TRX stain showed severe left ventricular hypertrophy on electrocardiogram and highly elevated left ventricular end-diastolic pressure (> 24 mmHg), suggesting the overload of oxidative stress by hemodynamic impairment. Myocardial TRX was upregulated in myocarditis and cardiomyopathies with active necrotic stage associated with DNA damage, which may reflect the oxidative stress overload in hemodynamically uncontrolled status. |
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Authors:
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Masaomi Nimata; Chiharu Kishimoto; Keisuke Shioji; Katsumi Ishizaki; Shoji Kitaguchi; Tetsuo Hashimoto; Norikazu Nagata; Chuichi Kawai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 248 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-21 Completed Date: 2004-04-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 193-6 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Biopsy Cardiomyopathies / metabolism* DNA / metabolism DNA Damage Deoxyguanosine / analogs & derivatives*, pharmacology Diastole Heart Ventricles / metabolism Humans Hypertrophy Immunohistochemistry Myocarditis / metabolism* Myocardium / metabolism* Necrosis Oxidation-Reduction* Oxidative Stress Pressure Thioredoxins / biosynthesis* Up-Regulation* |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyguanosine; 52500-60-4/Thioredoxins; 9007-49-2/DNA; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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