Document Detail

Upregulation of gp130 and STAT3 activation in the rat hippocampus following transient forebrain ischemia.
MedLine Citation:
PMID:  12528179     Owner:  NLM     Status:  MEDLINE    
To determine whether the pathophysiological processes after transient forebrain ischemia are mediated via a signal pathway involving gp130 (a signal transducer for the interleukin-6 family), we analyzed changes in the expression of gp130 and its downstream transcription factor, signal transducer and activator of transcription factor 3 (STAT3), in the rat hippocampus of a four-vessel occlusive ischemia model. Expression of gp130 mRNA was restricted to neurons of the pyramidal cell and granule cell layers in control animals. Four hours after ischemic injury, astrocytes expressed gp130 mRNA. Expression of gp130 increased preferentially in the CA1 and dentate hilar regions, and was maintained for at least 2 weeks. Increase in gp130 expression was accompanied by the activation of STAT3 following ischemic injury. Four hours after injury, STAT3 and phosphorylated STAT3 (pSTAT3) were observed in the nuclei of the dentate hilar region, and sequentially in the CA1 region at day 1. By day 3, STAT3 immunoreactivity markedly increased in these areas, where small cells with the morphology of astrocytes showed nuclear and cytoplasmic STAT3 and nuclear pSTAT3 immunoreactivities. These patterns were especially maintained in the CA1 area until 14 days of reperfusion. Double-labeling experiments revealed that the cells expressing STAT3 and pSTAT3 were glial fibrillary acidic protein-expressing reactive astrocytes. These results show a coordinated and long-lasting upregulation of gp130 mRNA and STAT3 activation in reactive astrocytes of the postischemic hippocampus, indicating that they may be involved in the astrocytic response to an ischemic insult.
Jeong-Sun Choi; Seong Yun Kim; Jung-Ho Cha; Yun-Sik Choi; Ki-Wug Sung; Seong Taek Oh; Ok Nyu Kim; Jin-Woong Chung; Myung-Hoon Chun; Sang Bok Lee; Mun-Yong Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  41     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-15     Completed Date:  2003-04-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-46     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Anatomy, College of Medicine, Catholic University of Korea, Seoul, Korea.
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MeSH Terms
Antigens, CD / genetics,  metabolism*
Astrocytes / metabolism*
Cytokine Receptor gp130
DNA-Binding Proteins / genetics,  metabolism*
Gene Expression / physiology
Hippocampus / cytology,  metabolism*
Ischemic Attack, Transient / metabolism*
Membrane Glycoproteins / genetics,  metabolism*
Pyramidal Cells / metabolism
RNA, Messenger / analysis
Rats, Sprague-Dawley
STAT3 Transcription Factor
Stroke / metabolism
Trans-Activators / genetics,  metabolism*
Up-Regulation / physiology
Reg. No./Substance:
0/Antigens, CD; 0/DNA-Binding Proteins; 0/Il6st protein, rat; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/STAT3 Transcription Factor; 0/Stat3 protein, rat; 0/Trans-Activators; 133483-10-0/Cytokine Receptor gp130

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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