Document Detail

Upregulation of coronary endothelial P-selectin in a monkey heart ischemia reperfusion model.
MedLine Citation:
PMID:  20803056     Owner:  NLM     Status:  MEDLINE    
The design of targeted ultrasound contrast agents for molecular imaging of myocardial ischemia-reperfusion (IR) requires the availability of an adequate in vivo model in a species in which cross reactivity with the target occurs. P-selectin (Psel) is an activation-dependent endothelial receptor that supports rapid and reversible cell adhesion in a flowing system. Together with E- and L-selectins it constitutes the selectin family of adhesion molecules. We investigated the myocardial expression of selectins in a closed chest minimally invasive monkey myocardial IR model. Catheter-based occlusion (30-50 min) followed by reperfusion (3 or 5 h) of left anterior descending artery (LAD) was performed in anesthetised monkeys. At the end of each procedure animals were killed, and their hearts were excised. The tissues were analyzed immunohistochemically using an anti-human Psel antibody (AK-6 clone) that cross reacts with rhesus monkey. Histopathological features confirm the presence of IR injuries in myocardial tissues. There was significant increase in the Psel expression in vessels from the IR areas. However, significantly higher Psel immunoreactivity was also seen in areas which are distant from IR injuries.
Regi Thomas; Yulan Cheng; Jun Yan; Thierry Bettinger; Anne Broillet; Gilles Rioufol; Adrian D Nunn
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Publication Detail:
Type:  Journal Article     Date:  2010-08-28
Journal Detail:
Title:  Journal of molecular histology     Volume:  41     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  277-87     Citation Subset:  IM    
Ernst Felder Laboratories, Discovery Biology, Bracco Research USA, 305 College Road East, Princeton, NJ 08540, USA.
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MeSH Terms
Antibody Specificity
Blood Platelets / metabolism
Cell Line
Cross Reactions
Disease Models, Animal
Endothelial Cells / metabolism,  pathology
Endothelium, Vascular / metabolism*,  pathology*
Lymphatic Vessels / metabolism
Myocardial Reperfusion Injury / metabolism*,  pathology*,  physiopathology
Myocardium / metabolism,  pathology
P-Selectin / metabolism*
Protein Transport
Reg. No./Substance:
0/Antibodies; 0/P-Selectin; 0/SELP protein, human

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