Document Detail

Upregulation of the cardiac homeobox gene Nkx2-5 (CSX) in feline right ventricular pressure overload.
MedLine Citation:
PMID:  9612365     Owner:  NLM     Status:  MEDLINE    
The recent characterization of the cardiac-specific homeobox gene Nkx2-5 (or CSX) and its detection in normal adult heart tissue raises the possibility of a role in adult hypertrophy. Using pressure overload as a primary stimulus, we used a feline pulmonary artery banding model to produce right ventricular hypertrophy (RVH). Total RNA was hybridized to a full-length murine Nkx2-5 cDNA probe that contained the NK family homeodomain. Nkx2-5 mRNA levels increased 5.1-fold (P < 0.05) and 3.9-fold vs. the corresponding left ventricles at 2 and 7 days of RVH, respectively, during the period of maximal myocardial growth. By 2 wk, when the RVH response had been completed, Nkx2-5 mRNA levels were returning toward baseline. Hybridization with an Nkx2-5 probe not containing the NK homologous homeodomain demonstrated that upregulation was specific for the Nkx2-5 gene. Atrial natriuretic factor and alpha-cardiac actin, both activated in part by Nkx2-5 DNA binding elements, also increased with RVH. These data suggest that a cardiac homeobox gene may play a role in the induction of adult cardiac hypertrophy.
J T Thompson; M S Rackley; T X O'Brien
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  274     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-24     Completed Date:  1998-06-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1569-73     Citation Subset:  IM    
Office of Research and Development, Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina, USA.
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MeSH Terms
Blood Pressure / genetics
Heart / physiopathology*
Homeodomain Proteins / genetics*
Hypertrophy, Right Ventricular / genetics*,  physiopathology*
Pulmonary Artery / pathology,  physiopathology
Transcription Factors*
Grant Support
Reg. No./Substance:
0/Homeodomain Proteins; 0/NKX2-5 protein, human; 0/Transcription Factors

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