| Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis. | |
| | |
MedLine Citation:
|
PMID: 16822877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. AIM: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. METHODS: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. RESULTS: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p=0.022). CONCLUSIONS: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC. |
| | |
Authors:
|
Hiu-Fung Yuen; Yuen-Piu Chan; Michelle Lok-Yee Wong; Wei-Kei Kwok; Ka-Kui Chan; Pin-Yin Lee; Gopesh Srivastava; Simon Ying-Kit Law; Yong-Chuan Wong; Xianghong Wang; Kwok-Wah Chan |
Related Documents
:
|
21330137 - Opposing oncogenic activities of small dna tumor virus transforming proteins. 11669287 - Tumor-associated antigen expression and growth requirements predict tumorigenesis in sq... 16043897 - Overexpression of phosphorylated-stat3 correlated with the invasion and metastasis of c... 9810887 - Occupationally related tumors in tar refinery workers. 22028757 - Surgical treatment of ventral and ventrolateral intradural extramedullary tumors of cra... 19111567 - Purification and characterization of a novel lectin from the toxic wild mushroom inocyb... |
Publication Detail:
|
Type: Journal Article Date: 2006-07-05 |
Journal Detail:
|
Title: Journal of clinical pathology Volume: 60 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2007 May |
Date Detail:
|
Created Date: 2007-05-21 Completed Date: 2007-06-28 Revised Date: 2010-09-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
|
Languages: eng Pagination: 510-4 Citation Subset: AIM; IM |
Affiliation:
|
Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Carcinoma, Squamous Cell / metabolism*, secondary, surgery Cell Transformation, Neoplastic / metabolism Esophageal Neoplasms / metabolism*, pathology, surgery Esophagectomy Female Gene Expression Regulation, Neoplastic Humans Male Middle Aged Neoplasm Proteins / biosynthesis, genetics Neoplasm Staging Nuclear Proteins / biosynthesis*, genetics Prognosis RNA, Messenger / genetics RNA, Neoplasm / genetics Reverse Transcriptase Polymerase Chain Reaction / methods Tumor Cells, Cultured Tumor Markers, Biological / biosynthesis*, genetics Twist Transcription Factor / biosynthesis*, genetics Up-Regulation* |
| Chemical | |
Reg. No./Substance:
|
0/Neoplasm Proteins; 0/Nuclear Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/TWIST1 protein, human; 0/Tumor Markers, Biological; 0/Twist Transcription Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Amplification of the HER2 gene in breast cancers testing 2+ weak positive by HercepTest immunohistoc...
Next Document: Utility of immunohistochemical analysis for cyclo-oxygenase 2 in the differential diagnosis of osteo...