Document Detail


Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis.
MedLine Citation:
PMID:  16822877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome. AIM: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC. METHODS: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR. RESULTS: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p<0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p=0.022). CONCLUSIONS: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.
Authors:
Hiu-Fung Yuen; Yuen-Piu Chan; Michelle Lok-Yee Wong; Wei-Kei Kwok; Ka-Kui Chan; Pin-Yin Lee; Gopesh Srivastava; Simon Ying-Kit Law; Yong-Chuan Wong; Xianghong Wang; Kwok-Wah Chan
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Publication Detail:
Type:  Journal Article     Date:  2006-07-05
Journal Detail:
Title:  Journal of clinical pathology     Volume:  60     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-06-28     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  510-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / metabolism*,  secondary,  surgery
Cell Transformation, Neoplastic / metabolism
Esophageal Neoplasms / metabolism*,  pathology,  surgery
Esophagectomy
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Middle Aged
Neoplasm Proteins / biosynthesis,  genetics
Neoplasm Staging
Nuclear Proteins / biosynthesis*,  genetics
Prognosis
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Reverse Transcriptase Polymerase Chain Reaction / methods
Tumor Cells, Cultured
Tumor Markers, Biological / biosynthesis*,  genetics
Twist Transcription Factor / biosynthesis*,  genetics
Up-Regulation*
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Nuclear Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/TWIST1 protein, human; 0/Tumor Markers, Biological; 0/Twist Transcription Factor
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