Document Detail


Upregulation of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E2 synthase-1 (mPGES-1) in wall of ruptured human cerebral aneurysms: preliminary results.
MedLine Citation:
PMID:  22588264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: Cyclooxygenase-2 (COX-2) and Microsomal Prostaglandin E2 Synthase-1 (mPGES-1) catalyze isomerization of the cyclooxygenase product PGH2 into PGE2. Deletion of COX-2/mPGES-1 suppresses carotid artery atherogenesis and angiotensin II-induced aortic aneurysms formation, and attenuates neointimal hyperplasia after vascular injury in mice. The upregulation of COX-2/mPGES-1 in the wall of ruptured human cerebral aneurysms is not known.
METHODS: Ten patients with intracranial aneurysms (5 ruptured and 5 nonruptured) underwent microsurgical clipping. During the procedure, a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for COX-1, COX-2, and mPGES-1. A segment of the superficial temporal artery was also removed and immunostained with monoclonal antibodies for COX-1, COX-2, and mPGES-1.
RESULTS: All 10 aneurysm tissues stained positive for mPGES-1 monoclonal antibody. Expression of mPGES-1 was more abundant in ruptured aneurysm tissue than in nonruptured aneurysms, based on a semiquantitative grading. None of the superficial temporal artery specimens expressed mPGES-1. COX-2 was upregulated in the same distribution as was mPGES-1. COX-1 was present constitutively in all tissues.
CONCLUSIONS: COX-2/mPGES-1 are expressed in the wall of human cerebral aneurysms and more abundantly so in ruptured aneurysms than in nonruptured. We speculate that the protective effect of aspirin against rupture of cerebral aneurysms may be mediated in part by inhibition of COX-2/mPGES-1.
Authors:
David Hasan; Tomoki Hashimoto; David Kung; R Loch Macdonald; H Richard Winn; Donald Heistad
Related Documents :
55524 - Ascending aorta-right pulmonary artery shunt.
21769824 - Congenital left main coronary artery aneurysm.
12556624 - Intracardiac echocardiography and transcranial doppler ultrasound to guide closure of p...
20144254 - A modelling study of atrial septostomy for pulmonary arterial hypertension, and its eff...
25489314 - Treatment of left main coronary artery stenosis with the stentys self-expandable drug-e...
877274 - The primary double-contrast examination of the postoperative stomach.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-15
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  43     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-26     Completed Date:  2012-09-04     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1964-7     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Carver College of Medicine, University of Iowa, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, JCP 1616, Iowa City, IA 52242, USA. david-hasan@uiowa.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aneurysm, Ruptured / enzymology*,  pathology
Cyclooxygenase 2 / biosynthesis*
Female
Humans
Intracranial Aneurysm / enzymology*,  pathology
Intramolecular Oxidoreductases / biosynthesis*
Male
Microsomes / enzymology*,  pathology
Middle Aged
Up-Regulation / physiology*
Grant Support
ID/Acronym/Agency:
24621//PHS HHS; HL62984/HL/NHLBI NIH HHS; R01NS055876/NS/NINDS NIH HHS; R03 NS079227/NS/NINDS NIH HHS; R03NS07922/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.99.3/prostaglandin-E synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Regional Variation in Recommended Treatments for Ischemic Stroke and TIA: Get With the Guidelines-St...
Next Document:  Differentiation of Transverse Sinus Thrombosis From Congenitally Atretic Cerebral Transverse Sinus W...