Document Detail


Upregulation of ATP-sensitive potassium channels for estrogen-mediated cell proliferation in human uterine leiomyoma cells.
MedLine Citation:
PMID:  18569028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The objectives of the present study were to evaluate the expression level of ATP-sensitive potassium (K(ATP)) channels in smooth muscle cells in human uterine leiomyoma and the involvement of the channel in potentiating effect of estrogen on leiomyoma growth. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR and Western blot were used for the identification and quantification of K(ATP)-channel subunits in the control myometrial and leiomyoma cells. Furthermore, we measured the K(ATP)-channel activity in enzymatically isolated single uterine smooth muscle cells by whole-cell patch-clamp recordings. The estrogen-induced cell proliferation in leiomyoma was measured by the MTT assay. RESULTS: The subunits of K(ATP) channels (Kir6.1, Kir6.2, SUR2B) were more highly expressed in leiomyoma cells than in control cells. The whole-cell currents mainly through K(ATP) channels were also greater in the leiomyoma cells. Estrogen applied in the bath solution could acutely enhance the channel activity. Estrogen-induced proliferation of the leiomyoma cells was inhibited by pretreatment with glibenclamide, a K(ATP)-channel inhibitor. CONCLUSION: Estrogen may induce the proliferation of leiomyoma cells, at least in part, by activating the K(ATP) channel. Increased expression of the K(ATP) channel may be a causal factor for the high growth rate of uterine leiomyoma.
Authors:
Sung-Hee Park; Sabarish Ramachandran; Sang-Hoon Kwon; Soon-Do Cha; Eul Won Seo; Insoo Bae; Chiheum Cho; Dae-Kyu Song
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology     Volume:  24     ISSN:  1473-0766     ISO Abbreviation:  Gynecol. Endocrinol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807913     Medline TA:  Gynecol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  250-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Growth Processes / physiology
Estradiol / pharmacology*
Estrogen Receptor alpha / biosynthesis,  genetics
Estrogen Receptor beta / biosynthesis,  genetics
Female
Formazans / chemistry
Glyburide / pharmacology
Humans
Immunoblotting
KATP Channels / biosynthesis*,  genetics
Leiomyoma / genetics,  metabolism*,  pathology
Middle Aged
Patch-Clamp Techniques
Pinacidil / pharmacology
RNA, Messenger / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Tetrazolium Salts / chemistry
Up-Regulation / drug effects
Uterine Neoplasms / genetics,  metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Formazans; 0/KATP Channels; 0/RNA, Messenger; 0/Tetrazolium Salts; 10238-21-8/Glyburide; 23305-68-2/MTT formazan; 50-28-2/Estradiol; 85371-64-8/Pinacidil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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