| Upregulation of ATP-sensitive potassium channels for estrogen-mediated cell proliferation in human uterine leiomyoma cells. | |
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MedLine Citation:
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PMID: 18569028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The objectives of the present study were to evaluate the expression level of ATP-sensitive potassium (K(ATP)) channels in smooth muscle cells in human uterine leiomyoma and the involvement of the channel in potentiating effect of estrogen on leiomyoma growth. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR and Western blot were used for the identification and quantification of K(ATP)-channel subunits in the control myometrial and leiomyoma cells. Furthermore, we measured the K(ATP)-channel activity in enzymatically isolated single uterine smooth muscle cells by whole-cell patch-clamp recordings. The estrogen-induced cell proliferation in leiomyoma was measured by the MTT assay. RESULTS: The subunits of K(ATP) channels (Kir6.1, Kir6.2, SUR2B) were more highly expressed in leiomyoma cells than in control cells. The whole-cell currents mainly through K(ATP) channels were also greater in the leiomyoma cells. Estrogen applied in the bath solution could acutely enhance the channel activity. Estrogen-induced proliferation of the leiomyoma cells was inhibited by pretreatment with glibenclamide, a K(ATP)-channel inhibitor. CONCLUSION: Estrogen may induce the proliferation of leiomyoma cells, at least in part, by activating the K(ATP) channel. Increased expression of the K(ATP) channel may be a causal factor for the high growth rate of uterine leiomyoma. |
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Authors:
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Sung-Hee Park; Sabarish Ramachandran; Sang-Hoon Kwon; Soon-Do Cha; Eul Won Seo; Insoo Bae; Chiheum Cho; Dae-Kyu Song |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology Volume: 24 ISSN: 1473-0766 ISO Abbreviation: Gynecol. Endocrinol. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-06-23 Completed Date: 2008-09-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807913 Medline TA: Gynecol Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 250-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, Keimyung University School of Medicine, Daegu, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Growth Processes / physiology Estradiol / pharmacology* Estrogen Receptor alpha / biosynthesis, genetics Estrogen Receptor beta / biosynthesis, genetics Female Formazans / chemistry Glyburide / pharmacology Humans Immunoblotting KATP Channels / biosynthesis*, genetics Leiomyoma / genetics, metabolism*, pathology Middle Aged Patch-Clamp Techniques Pinacidil / pharmacology RNA, Messenger / biosynthesis, genetics Reverse Transcriptase Polymerase Chain Reaction Tetrazolium Salts / chemistry Up-Regulation / drug effects Uterine Neoplasms / genetics, metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Formazans; 0/KATP Channels; 0/RNA, Messenger; 0/Tetrazolium Salts; 10238-21-8/Glyburide; 23305-68-2/MTT formazan; 50-28-2/Estradiol; 85371-64-8/Pinacidil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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