Document Detail


Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador.
MedLine Citation:
PMID:  23071640     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Observations that the airway microbiome is disturbed in asthma may be confounded by the widespread use of antibiotics and inhaled steroids. We have therefore examined the oropharyngeal microbiome in early onset wheezing infants from a rural area of tropical Ecuador where antibiotic usage is minimal and glucocorticoid usage is absent.
MATERIALS AND METHODS: We performed pyrosequencing of amplicons of the polymorphic bacterial 16S rRNA gene from oropharyngeal samples from 24 infants with non-infectious early onset wheezing and 24 healthy controls (average age 10.2 months). We analyzed microbial community structure and differences between cases and controls by QIIME software.
RESULTS: We obtained 76,627 high quality sequences classified into 182 operational taxonomic units (OTUs). Firmicutes was the most common and diverse phylum (71.22% of sequences) with Streptococcus being the most common genus (49.72%). Known pathogens were found significantly more often in cases of infantile wheeze compared to controls, exemplified by Haemophilus spp. (OR=2.12, 95% Confidence Interval (CI) 1.82-2.47; P=5.46×10(-23)) and Staphylococcus spp. (OR=124.1, 95%CI 59.0-261.2; P=1.87×10(-241)). Other OTUs were less common in cases than controls, notably Veillonella spp. (OR=0.59, 95%CI=0.56-0.62; P=8.06×10(-86)).
DISCUSSION: The airway microbiota appeared to contain many more Streptococci than found in Western Europe and the USA. Comparisons between healthy and wheezing infants revealed a significant difference in several bacterial phylotypes that were not confounded by antibiotics or use of inhaled steroids. The increased prevalence of pathogens such as Haemophilus and Staphylococcus spp. in cases may contribute to wheezing illnesses in this age group.
Authors:
Paul Andres Cardenas; Philip J Cooper; Michael J Cox; Martha Chico; Carlos Arias; Miriam F Moffatt; William Osmond Cookson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-05
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-16     Completed Date:  2013-04-11     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e46803     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  genetics*
Case-Control Studies
Cohort Studies
DNA, Bacterial / chemistry,  genetics
Ecuador
Female
Genetic Variation
Haemophilus / classification,  genetics
Humans
Infant
Male
Metagenome / genetics*
Oropharynx / microbiology*
Phylogeny
RNA, Ribosomal, 16S / genetics
Respiratory Sounds / diagnosis*
Rural Health / statistics & numerical data
Sequence Analysis, DNA / methods
Species Specificity
Staphylococcus / classification,  genetics
Streptococcus / classification,  genetics
Tropical Climate
Grant Support
ID/Acronym/Agency:
088862//Wellcome Trust; 088862/Z/09/Z//Wellcome Trust; 097117//Wellcome Trust
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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