Document Detail

Up-regulation of microsomal prostaglandin E synthase-1 in COX-1 and COX-2 knock-out mouse fibroblast cell lines.
MedLine Citation:
PMID:  19114118     Owner:  NLM     Status:  MEDLINE    
In this paper we investigated the possible involvement of prostaglandin E synthases (PGESs) in compensatory mechanism. Our findings showed that microsomal (m)PGES-1 expression was significantly up-regulated in COX knock-out (K/O) cells whereas the expression of cytosolic PGES was not changed indicating that the induction of mPGES-1 may, at least in part, contribute to the substantial increase of PGE(2) production in COX K/O cell lines. The selective up-regulation of mPGES-1 in COX-2 K/O cells suggests that mPGES-1 may be metabolically coupled with COX-1 for PGE(2) formation. Addition of arachidonic acid caused significant induction of mPGES-1 and COX-2 in WT cells, whereas COX-1 and cPGES were not affected. Our earlier and the current studies demonstrate the coregulation of cPLA(2), COX, and mPGES-1, in PGE(2) synthesis pathway, and that these enzymes contribute to the elevation of PGE(2) level when one COX isoform is absent.
Duanpen Sandee; Sasitorn Sivanuntakorn; Vanicha Vichai; Jarin Kramyu; Kanyawim Kirtikara
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Publication Detail:
Type:  Journal Article     Date:  2008-12-09
Journal Detail:
Title:  Prostaglandins & other lipid mediators     Volume:  88     ISSN:  1098-8823     ISO Abbreviation:  Prostaglandins Other Lipid Mediat.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-30     Completed Date:  2009-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9808648     Medline TA:  Prostaglandins Other Lipid Mediat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  111-6     Citation Subset:  IM    
The National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani 12120, Thailand.
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MeSH Terms
Arachidonic Acid / pharmacology
Blotting, Western
Cell Line
Cyclooxygenase 1 / genetics*
Cyclooxygenase 2 / genetics*
Fibroblasts / cytology*,  metabolism*
Intramolecular Oxidoreductases / genetics,  metabolism*
Mice, Knockout
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation* / drug effects
Reg. No./Substance:
506-32-1/Arachidonic Acid; EC 1.14.99.-/Ptgs2 protein, mouse; EC 1; EC 2; EC 5.3.-/Intramolecular Oxidoreductases; EC synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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