Document Detail


Up-regulation of lysozyme production in colonic adenomas and adenocarcinomas.
MedLine Citation:
PMID:  9543668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The presence of lysozyme protein in some gastric adenomas and adenocarcinomas has been well documented. There have been relatively few studies investigating the presence of lysozyme in tumours of the large intestine and they show contrasting results. We aim to investigate the cellular source and expression of lysozyme in colonic adenomas and adenocarcinomas. METHODS AND RESULTS: We randomly selected 29 and 27 colonic adenomas and adenocarcinomas, respectively. Using in-situ hybridization (ISH) and immunohistochemistry (IHC), we found an up-regulation of lysozyme in the dysplastic epithelium of all the adenomas studied, with more than 80% of cases expressing moderate to strong signals. Although the up-regulation of lysozyme was also observed in adenocarcinomas, only 30% of the cases showed moderate to strong signals, mostly with an uneven distribution. Down-regulation of lysozyme in the severely dysplastic and invasive foci were noted in some cases of adenoma with malignant transformation. Normal colonic glands were consistently negative for lysozyme at both the mRNA and the protein level, but inflamed and immature regenerative colonic epithelium at the crypt base showed positive signals in a similar pattern to those observed in the dysplastic epithelium of the adenomas. CONCLUSIONS: Our results confirm that colonic epithelium can produce lysozyme and its expression is up-regulated in the dysplastic epithelium in adenomas and in invasive cancer cells. It is interesting that regenerative colonic epithelium showed a similar pattern of lysozyme expression as in adenomas. The loss of lysozyme secreting phenotype in most of the invasive tumours suggests that lysozyme may not confer an advantage to tumour progression.
Authors:
S T Yuen; M P Wong; L P Chung; S Y Chan; N Cheung; J Ho; S Y Leung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histopathology     Volume:  32     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-06-01     Completed Date:  1998-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  126-32     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology*,  pathology
Adenoma / enzymology*,  pathology
Colonic Neoplasms / enzymology*,  pathology
Humans
Immunohistochemistry
In Situ Hybridization
Intestinal Mucosa / enzymology,  pathology
Muramidase / biosynthesis*,  genetics
RNA, Messenger / biosynthesis
Random Allocation
Up-Regulation*
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.2.1.17/Muramidase

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