Document Detail

Up-regulation of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in sentinel lymph nodes of colorectal cancer.
MedLine Citation:
PMID:  20872967     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the early metastasis-associated proteins in sentinel lymph node micrometastasis (SLNMM) of colorectal cancer (CRC) through comparative proteome.
METHODS: Hydrophobic protein samples were extracted from individual-matched normal lymph nodes (NLN) and SLNMM of CRC. Differentially expressed protein spots were detected by two-dimensional electrophoresis and image analysis, and subsequently identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry-mass spectrometry and Western blotting, respectively.
RESULTS: Forty proteins were differentially expressed in NLN and SLNMM, and 4 metastasis-concerned proteins highly expressed in SLNMM were identified to be hnRNP A1, Ezrin, tubulin β-2C and Annexin A1. Further immunohistochemistry staining of these four proteins showed their clinicopathological characteristics in lymph node metastasis of CRC.
CONCLUSION: Variations of hydrophobic protein expression in NLN and SLNMM of CRC and increased expression of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in SLNMM suggest a significantly elevated early CRC metastasis.
Zhen-Yu He; Hao Wen; Chuan-Bing Shi; Jie Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-28     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4670-6     Citation Subset:  IM    
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MeSH Terms
Aged, 80 and over
Annexin A1 / metabolism*
Biological Markers / metabolism
Colorectal Neoplasms* / metabolism,  pathology,  surgery
Cytoskeletal Proteins / metabolism*
Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism*
Lymph Nodes / metabolism*,  pathology
Lymphatic Metastasis
Middle Aged
Molecular Sequence Data
Protein Isoforms / metabolism*
Proteome / analysis
Sentinel Lymph Node Biopsy
Tubulin / metabolism*
Reg. No./Substance:
0/Annexin A1; 0/Biological Markers; 0/Cytoskeletal Proteins; 0/Heterogeneous-Nuclear Ribonucleoprotein Group A-B; 0/Protein Isoforms; 0/Proteome; 0/Tubulin; 0/ezrin; 0/hnRNP A1

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