| Up-regulation of hepatic lipolysis stimulated lipoprotein receptor by leptin: a potential lever for controlling lipid clearance during the postprandial phase. | |
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MedLine Citation:
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PMID: 20647547 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As a hepatic receptor for triglyceride-rich lipoproteins, the lipolysis-stimulated lipoprotein receptor (LSR) may be involved in the dynamics of lipid distribution between the liver and peripheral tissues. Here, we explore the potential role of leptin in regulating LSR. At physiological concentrations (1-10 ng/ml), leptin increased LSR protein and mRNA levels in Hepa1-6 cells through an ERK1/2-dependent and α-amanitin-sensitive pathway. In vivo, leptin treatment of C57BL6/Rj mice (1 μg 2×/d, 8 d) led to a significant increase in hepatic LSR mRNA and protein, decreased liver triglycerides and increased VLDL secretion as compared to controls. LSR(+/-) mice with elevated postprandial lipemia placed on a high-fat (60% kcal) diet exhibited accelerated weight gain and increased fat mass as compared to controls. While plasma leptin levels were increased 3-fold, hepatic leptin receptor protein levels and phosphorylation of ERK1/2 were significantly reduced. Therefore, leptin is an important regulator of LSR protein levels providing the means for the control of hepatic uptake of lipids during the postprandial phase. However, this may no longer be functional in LSR(+/-) mice placed under a chronic dietary fat load, suggesting that this animal model could be useful for the study of molecular mechanisms involved in peripheral leptin resistance. |
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Authors:
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Christophe Stenger; Marine Hanse; Dagmar Pratte; Marie-Ludvine Mbala; Samina Akbar; Violette Koziel; Marie-Christine Escanyé; Badreddine Kriem; Catherine Malaplate-Armand; Jean-Luc Olivier; Thierry Oster; Thierry Pillot; Frances T Yen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-20 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 24 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: 2010-12-21 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 4218-28 Citation Subset: IM |
Affiliation:
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Lipidomix, Nancy University, Institut National Polytechnique de Lorraine-Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires (INPL ENSAIA), Vandoeuvre-lès-Nancy, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Body Weight / drug effects Cell Line Fluorescent Antibody Technique Leptin / blood, pharmacology* Lipid Metabolism / drug effects* Lipolysis / drug effects* Liver / drug effects*, metabolism Mice Mice, Inbred C57BL Polymerase Chain Reaction Postprandial Period* Receptors, Lipoprotein / metabolism* Up-Regulation / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Leptin; 0/Receptors, Lipoprotein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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