| Up-regulation of expression of interferon-stimulated gene 15 in the bovine corpus luteum during early pregnancy. | |
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MedLine Citation:
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PMID: 20172220 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interferon-tau (IFNT), the pregnancy recognition signal in ruminant species, is secreted by conceptus trophectoderm cells and induces expression of IFN-stimulated gene 15 (ISG15) in the uterus and corpus luteum (CL) in ewes. Expression of ISG15 in ovine CL is speculated to be through an endocrine pathway, but it is unclear whether expression of ISG15 in bovine CL is via such a pathway. In this study, CL were obtained from cows on d 16, 25, 60, 120, 180, and 270 of pregnancy, and endometrium, mammary gland, ovarian stroma, and CL were also collected from cows on d 18 of pregnancy and on d 15 and 18 of the estrous cycle. All tissue explants from d 15 of the estrous cycle were cultured in the absence or presence of 100ng/mL of recombinant bovine IFNT for 24h. The results indicated that ISG15 and conjugated proteins were expressed in CL of both cyclic and pregnant cows regardless of pregnancy status and were upregulated during early pregnancy. The mammary gland from d 18 of pregnancy did not express ISG15, but explants of the mammary gland from d 15 of the estrous cycle did express ISG15 after being treated with IFNT. However, luteal explants from d 15 of the estrous cycle did not express ISG15 after being cultured for 24h. In conclusion, ISG15 expression is upregulated in the bovine CL during early pregnancy. Interestingly, cultured CL cells do not respond to IFNT, suggesting that the pregnancy-dependent stimulation of ISG15 expression is controlled by something other than IFNT in the bloodstream. |
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Authors:
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L Yang; X L Wang; P C Wan; L Y Zhang; Y Wu; D W Tang; S M Zeng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-04-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1000-11 Citation Subset: IM |
Affiliation:
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College of Animal Science and State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antiviral Agents / pharmacology Cattle / metabolism, physiology* Corpus Luteum / drug effects, metabolism* Female Gene Expression Profiling Interferons / pharmacology Mammary Glands, Animal / drug effects, metabolism* Ovary / drug effects, metabolism Pregnancy Pregnancy, Animal* Up-Regulation* / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 9008-11-1/Interferons |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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