| Up-regulation of the endothelin receptor A in placental tissue from first trimester delayed miscarriages. | |
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MedLine Citation:
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PMID: 16879994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: This study tested the hypothesis that the endothelin (ET)/ET receptor (ETR) system in biologic fluids and in the human placenta is altered in delayed miscarriages as compared to apparently normal early pregnancies (reference group). METHODS: Immunoreactive ET (irET) concentrations were measured in plasma, urine, and cervical smears from 57 pregnant women in the weeks 6 to 14 of gestation (46 delayed miscarriages, 11 references) with radioimmunoassay (RIA). ET-1, ETR-A, and ETR-B mRNA, and ETR protein expression were measured in placental tissue of 45 early pregnancies (31 delayed miscarriages, 14 references) using semiquantitative reverse-transcription polymerase chain reaction (RT-PCR) and immunoblotting, respectively. RESULTS: irET levels in plasma, urine, and cervical smears did not differ between groups. Two prevailing ETR-A and ETR-B proteins were found at 45 and 55 kd, and were distributed similarly in delayed miscarriages and references. ETR-A protein and mRNA levels were 54% (P = .009) and threefold (P = .021) higher, respectively, in delayed miscarriages versus references. There was no difference in placental ETR-B and ET-1 mRNA levels between groups. CONCLUSION: Neither irET nor ET-1 mRNA levels differ between delayed miscarriages and normal early pregnancies. Pregnancies at risk for miscarriage cannot be identified by measurement of ET in plasma, urine, or cervical smears. Within the ET/ETR system, ETR-A is selectively up-regulated in placental tissue of delayed miscarriages as compared to normal pregnancies. ETR protein processing is similar in both groups. |
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Authors:
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Martina Dieber-Rotheneder; Christina Stern; Gernot Desoye; Mila Cervar-Zivkovic |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-31 |
Journal Detail:
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Title: Journal of the Society for Gynecologic Investigation Volume: 13 ISSN: 1556-7117 ISO Abbreviation: J. Soc. Gynecol. Investig. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-01 Completed Date: 2006-10-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9433806 Medline TA: J Soc Gynecol Investig Country: United States |
Other Details:
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Languages: eng Pagination: 435-41 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynaecology, Medical University of Graz, Graz, Austria. martina.dieber@meduni-graz.at |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abortion, Spontaneous
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metabolism* Adult Blotting, Western Electrophoresis, Polyacrylamide Gel Endothelin-1 / analysis, genetics Endothelins / analysis, blood, urine Female Gestational Age Humans Placenta / chemistry* Pregnancy RNA, Messenger / analysis Receptor, Endothelin A / analysis, genetics* Receptor, Endothelin B / analysis, genetics Reverse Transcriptase Polymerase Chain Reaction Up-Regulation Vaginal Smears |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Endothelins; 0/RNA, Messenger; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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