Document Detail


Up-regulated miR-17 promotes cell proliferation, tumor growth and cell cycle progression by targeting RND3 tumor suppressor gene in colorectal carcinoma.
MedLine Citation:
PMID:  22132820     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Emerging evidence indicates that the miR-17 family may have a causal role in human cancer tumorigenesis, but their specific effects on occurrence of colorectal carcinoma (CRC) are still poorly understood. In this study, we profiled CRC tissue samples by miRNA microarray and found that four members of the miR-17 family had higher expressions in CRC tissues than in normal tissues. This finding was further validated by qRT-PCR. Transfecting CRC cells with an inhibitor of miR-17 lowered their ability to proliferate and induced G0/G1 arrest. We also confirmed that miR-17 exerted this function by directly targeting RND3 in vitro and that the expression of miR-17 was negatively correlated with that of RND3 in CRC tissues and CRC cells. Moreover, miR-17 inhibition led to tumor growth suppression and up-regulation of RND3 expression in a nude mouse xenograft model. RND3 expression was found significantly lower in CRC tissues than in normal tissues and adenomas, indicating RND3 may act as a tumor suppressor gene in CRC. In conclusion, our study suggests that miR-17 plays an important role in CRC carcinogenesis by targeting RND3 and may be a therapeutic agent for CRC.
Authors:
Hesan Luo; Jinjin Zou; Zhongyi Dong; Qin Zeng; Dehua Wu; Li Liu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-1
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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