| Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition. | |
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MedLine Citation:
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PMID: 20467341 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The aim of this study was to examine correlations of the islet-specific microRNA-375 expression to islet amyloid formation and pancreatic islet damage in human type 2 diabetes. METHODS: Autopsy pancreas samples from 40 type 2 diabetic and 15 nondiabetic patients were used to detect microRNA-375 expression using real-time quantitative polymerase chain reaction. Serial paraffin sections of the corresponding type 2 diabetic and nondiabetic cases were stained by immunofluorescence to evaluate for amylin expression, amyloid formation, and proportions of alpha and beta cells. RESULTS: Pancreatic microRNA-375 expression was increased in type 2 diabetic patients comparing with the nondiabetic patients (median, 4.02 for the diabetic patients vs 0.92 for the nondiabetic patients; P = 0.0001). The median was 6.14 for the diabetic patients with islet amyloid and 3.51 for islet amyloid-free diabetic patients. The expression level of microRNA-375 correlated positively with the frequency and the severity of islet amyloid formation and negatively with proportions of islet beta-cells and amylin-positive area, and islet mitochondria density. CONCLUSIONS: Up-regulated microRNA-375 is associated with type 2 diabetes and pancreatic islet amyloid formation and beta-cell deficit. microRNA-375 may serve as a biomarker for known and novel pathways in the pathogenesis of type 2 diabetes related to islet amyloid deposition and beta-cell dysfunction. |
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Authors:
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Hailu Zhao; Jing Guan; Heung-Man Lee; Yi Sui; Lan He; Jennifer J Siu; Patty P P Tse; Peter C Y Tong; Fernand M M Lai; Juliana C N Chan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pancreas Volume: 39 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2011-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
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Languages: eng Pagination: 843-6 Citation Subset: IM |
Affiliation:
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Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Amyloid / metabolism Autopsy Diabetes Mellitus, Type 2 / genetics*, metabolism, physiopathology Female Humans Insulin-Secreting Cells / metabolism* Islet Amyloid Polypeptide / metabolism Islets of Langerhans / metabolism*, physiopathology Male MicroRNAs / genetics* Microscopy, Fluorescence Middle Aged Reverse Transcriptase Polymerase Chain Reaction Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Amyloid; 0/Islet Amyloid Polypeptide; 0/MIRN375 microRNA, human; 0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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