| Untranslated regions of FbRbcS1 mRNA mediate bundle sheath cell-specific gene expression in leaves of a C4 plant. | |
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MedLine Citation:
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PMID: 16803877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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C4 photosynthesis typically requires two specialized leaf cell types, bundle sheath (bs) and mesophyll (mp), which provide the foundation for this highly efficient carbon assimilation pathway. In leaves of Flaveria bidentis, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (rubisco) accumulates only in bs cells surrounding the vascular centers and not in mp cells. This is in contrast to the more common C3 plants, which accumulate rubisco in all photosynthetic cells. Many previous studies have focused on transcriptional control of C4 cell type-specificity; however, post-transcriptional regulation has also been implicated in the bs-specific expression of genes encoding the rubisco subunits. In this current study, a biolistic leaf transformation assay has provided direct evidence that the 5'- and 3'-untranslated regions (UTRs) of F. bidentis FbRbcS1 mRNA (from a nuclear gene encoding the rubisco small subunit), in themselves, confer strong bs cell-specific expression to gfpA reporter gene transcripts when transcribed from a constitutive CaMV promoter. In transformed leaf regions, strong bs cell-specific GFP expression was accompanied by corresponding bs cell-specific accumulation of the constitutively transcribed FbRbcS1 5'-UTR-gfpA-3'-UTR mRNAs. Control constructs lacking any RbcS mRNA sequences were expressed in all leaf cell types. These findings demonstrate that characteristic cell type-specific FbRbcS1 expression patterns in C4 leaves can be established entirely by sequences contained within the transcribed UTRs of FbRbcS1 mRNAs. We conclude that selective transcript stabilization (in bs cells) or degradation (in mp cells) plays a key role in determining bs cell-specific localization of the rubisco enzyme. |
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Authors:
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Minesh Patel; Alan J Siegel; James O Berry |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-06-27 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 281 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-28 Completed Date: 2006-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 25485-91 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, State University of New York, Buffalo, New York 14260, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon
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chemistry Cell Nucleus / metabolism Flaveria / metabolism* Gene Expression Regulation, Plant* Genetic Vectors Humans In Situ Hybridization Microscopy, Fluorescence Photosynthesis Plant Leaves / metabolism RNA Processing, Post-Transcriptional* RNA, Messenger / metabolism* Ribulose-Bisphosphate Carboxylase / chemistry |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 7440-44-0/Carbon; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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