| Unstable Escherichia coli L forms revisited: growth requires peptidoglycan synthesis. | |
| | |
MedLine Citation:
|
PMID: 17586646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Growing bacterial L forms are reputed to lack peptidoglycan, although cell division is normally inseparable from septal peptidoglycan synthesis. To explore which cell division functions L forms use, we established a protocol for quantitatively converting a culture of a wild-type Escherichia coli K-12 strain overnight to a growing L-form-like state by use of the beta-lactam cefsulodin, a specific inhibitor of penicillin-binding proteins (PBPs) 1A and 1B. In rich hypertonic medium containing cefsulodin, all cells are spherical and osmosensitive, like classical L forms. Surprisingly, however, mutant studies showed that colony formation requires d-glutamate, diaminopimelate, and MurA activity, all of which are specific to peptidoglycan synthesis. High-performance liquid chromatography analysis confirmed that these L-form-like cells contain peptidoglycan, with 7% of the normal amount. Moreover, the beta-lactam piperacillin, a specific inhibitor of the cell division protein PBP 3, rapidly blocks the cell division of these L-form-like cells. Similarly, penicillin-induced L-form-like cells, which grow only within the agar layers of rich hypertonic plates, also require d-glutamate, diaminopimelate, and MurA activity. These results strongly suggest that cefsulodin- and penicillin-induced L-form-like cells of E. coli-and possibly all L forms-have residual peptidoglycan synthesis which is essential for their growth, probably being required for cell division. |
| | |
Authors:
|
Danièle Joseleau-Petit; Jean-Claude Liébart; Juan A Ayala; Richard D'Ari |
Related Documents
:
|
19350986 - Escherichia coli thermal inactivation relative to physiological state. 11292766 - Escherichia coli cdtb mediates cytolethal distending toxin cell cycle arrest. 2644216 - Apparent minimal size required for cell division in escherichia coli. 20615986 - Asymmetry in the clockwise and counterclockwise rotation of the bacterial flagellar motor. 19507186 - Radiosensitization of ht-29 cells and xenografts by the nitric oxide donor detanonoate. 8853796 - Interaction between cell-surface-expressed 70-kilodalton heat shock protein-like antige... |
Publication Detail:
|
Type: Journal Article Date: 2007-06-22 |
Journal Detail:
|
Title: Journal of bacteriology Volume: 189 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-09-03 Completed Date: 2007-10-19 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
|
Languages: eng Pagination: 6512-20 Citation Subset: IM |
Affiliation:
|
Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France. dari@ijm.jussieu.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
pharmacology Cefsulodin / pharmacology Cell Division Cell Wall / chemistry Culture Media Escherichia coli / drug effects, genetics, growth & development*, metabolism L Forms / drug effects, growth & development* Mutation Peptidoglycan / biosynthesis* Piperacillin / pharmacology beta-Lactams / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Culture Media; 0/Peptidoglycan; 0/beta-Lactams; 61477-96-1/Piperacillin; 62587-73-9/Cefsulodin |
| Comments/Corrections | |
Comment In:
|
J Bacteriol. 2007 Sep;189(18):6509-11
[PMID:
17644588
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ler and H-NS, regulators controlling expression of the long polar fimbriae of Escherichia coli O157:...
Next Document: Membrane sequestration of PII proteins and nitrogenase regulation in the photosynthetic bacterium Rh...