Document Detail


Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators.
MedLine Citation:
PMID:  22105830     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mesenchymal stem cells (MSC) represent emerging cell-based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non-healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability of these multipotent mesenchymal stromal cells to release soluble mediators that reduce inflammation and promote both angiogenesis and cell survival at sites of tissue damage. Growing evidence suggests that MSC secretion of soluble factors is dependent on tissue microenvironment. Despite the contribution of fatty acids to the metabolic environment of type 2 diabetes, almost nothing is known about their effects on MSC secretion of growth factors and cytokines. In this study, human bone marrow-derived MSC were exposed to linoleic acid, an omega-6 polyunsaturated fatty acid, or oleic acid, a monounsaturated fatty acid, for seven days in the presence of 5.38 mM glucose. Outcomes measured included MSC proliferation, gene expression, protein secretion and chemotaxis. Linoleic and oleic acids inhibited MSC proliferation and altered MSC expression and secretion of known mediators of angiogenesis. Both unsaturated fatty acids induced MSC to increase secretion of interleukin-6, VEGF and nitric oxide. In addition, linoleic acid but not oleic acid induced MSC to increase production of interleukin-8. Collectively these data suggest that exposure to fatty acids may have functional consequences for MSC therapy. Fatty acids may affect MSC engraftment to injured tissue and MSC secretion of cytokines and growth factors that regulate local cellular responses to injury.
Authors:
Andria N Smith; Lara A Muffley; Austin N Bell; Surawej Numhom; Anne M Hocking
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  227     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-05-21     Completed Date:  2012-07-25     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3225-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Angiogenic Proteins / genetics,  metabolism*
Bone Marrow / drug effects,  metabolism*
Cell Count
Cell Proliferation / drug effects
Cell- and Tissue-Based Therapy / methods
Chemotaxis / drug effects
Gene Expression / drug effects
Glucose / pharmacology
Humans
Linoleic Acid / pharmacology*
Mesenchymal Stromal Cells / drug effects,  metabolism*
Nitric Oxide / metabolism
Oleic Acid / pharmacology*
Wound Healing*
Grant Support
ID/Acronym/Agency:
P30 DK-17047/DK/NIDDK NIH HHS; P30 DK017047-34/DK/NIDDK NIH HHS; P40 OD011050/OD/NIH HHS; P40 RR-017447/RR/NCRR NIH HHS; P40 RR017447-07/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Angiogenic Proteins; 2UMI9U37CP/Oleic Acid; 31C4KY9ESH/Nitric Oxide; 9KJL21T0QJ/Linoleic Acid; IY9XDZ35W2/Glucose
Comments/Corrections

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