Document Detail

Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae.
MedLine Citation:
PMID:  18508626     Owner:  NLM     Status:  MEDLINE    
Condensin is the core activity responsible for chromosome condensation in mitosis. In the yeast S. cerevisiae, condensin binding is enriched at the regions where DNA replication terminates. Therefore, we investigated whether DNA replication completion determines the condensin-binding proficiency of chromatin. In order to fulfill putative mitotic requirements for condensin activity we analyzed chromosome condensation and condensin binding to unreplicated chromosomes in mitosis. For this purpose we used pGAL:CDC6 cdc15-ts cells that are known to enter mitosis without DNA replication if CDC6 transcription is repressed prior to S-phase. Both the condensation of nucleolar chromatin and proper condensin targeting to rDNA sites failed when unreplicated chromosomes were driven in mitosis. We propose that the DNA replication results in structural and/or biochemical changes to replicated chromatin, which are required for two-phase condensin binding and proper chromosome condensation.
Stanimir Dulev; Luis Aragon; Alexander Strunnikov
Related Documents :
15753656 - Dna repair in the context of chromatin.
20197036 - Transcription within condensed chromatin: steric hindrance facilitates elongation.
6180606 - Role of chromatin phospholipids on template availability and ultrastructure of isolated...
11140636 - A role for saccharomyces cerevisiae histone h2a in dna repair.
2671036 - Method for improved extraction of dna from nocardia asteroides.
19269326 - Lux ex tenebris: nucleotide resolution dna repair and nucleosome mapping.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2008-05-01
Journal Detail:
Title:  Frontiers in bioscience : a journal and virtual library     Volume:  13     ISSN:  1093-9946     ISO Abbreviation:  Front. Biosci.     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-29     Completed Date:  2008-09-19     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9709506     Medline TA:  Front Biosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5838-46     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine Triphosphatases / metabolism
Chromosomes, Fungal / genetics,  physiology*
DNA Replication
DNA, Fungal / genetics*,  metabolism
DNA, Ribosomal / genetics,  metabolism
DNA-Binding Proteins / metabolism
Mitosis / physiology*
Multiprotein Complexes / metabolism
Saccharomyces cerevisiae / genetics*,  physiology*
Saccharomyces cerevisiae Proteins / metabolism
Grant Support
MC_U120074328//Medical Research Council; Z01 HD001903-11/HD/NICHD NIH HHS; Z99 AI999999/AI/NIAID NIH HHS; //Medical Research Council
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Ribosomal; 0/DNA-Binding Proteins; 0/Multiprotein Complexes; 0/Saccharomyces cerevisiae Proteins; 0/condensin complexes; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Pancreatic acinar-to-beta cell transdifferentiation in vitro.
Next Document:  The tumor antigen EpCAM: tetraspanins and the tight junction protein claudin-7, new partners, new fu...