| Unmethylated CpG oligodeoxynucleotides activate head kidney leukocytes of Atlantic cod, Gadus morhua. | |
| | |
MedLine Citation:
|
PMID: 20349339 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Bacterial DNA and synthetic oligodeoxynucleotides (ODNs) that contain unmethylated CpG motifs are strong inducers of immune response in most mammalian organisms. The use of these synthetic CpG motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system. However, much less is known in other species of fish such as gadoids including Atlantic cod, Gadus morhua. Using head kidney (HK) leukocytes of cod in an in vitro study, we determined the effects of some established CpG-ODNs on the cellular responses of the fish immunocytes. Incubation of the HK leukocytes with 2 μM concentration of the CpG-ODNs resulted in enhanced respiratory burst. There were differential effects on the activities of acid phosphatase and cellular myeloperoxidase. Only CpG-ODN 1826 triggered a significant increase in the level of both enzymes. On the other hand, the supernatants derived from the HK leukocytes after incubation with different CpG-ODNs did not possess bactericidal activity against Vibrio anguillarum and Aeromonas salmonicida. This study has shown that CpG-ODNs at low concentrations are able to stimulate respiratory burst in cod but have minimal effects on cellular enzymatic activities and antibacterial action. |
| | |
Authors:
|
Christopher Marlowe A Caipang; Sanchala Gallage; Carlo C Lazado; Monica F Brinchmann; Viswanath Kiron |
Related Documents
:
|
10930299 - Il-12 elispot assays to detect and enumerate il-12 secreting cells. 8946679 - Inhibition of the human allogeneic mixed lymphocyte response by cyclosporin a: relation... 15383579 - Cpg dna induces igg class switch dna recombination by activating human b cells through ... 19161429 - Interleukin-35: odd one out or part of the family? 18300039 - Quantitative evaluation of interleukin-12 p40 gene expression in peripheral blood monon... 15364109 - Cell mediated immune response elicited in mice after immunization with the p64k meningo... 22626939 - Biodistribution and inflammatory profiles of novel penton and hexon double-mutant serot... 3950179 - Hyperinflammatory proliferative (blastomycosis-like) pyodermas: review, mechanisms, and... 9730899 - Differential regulation and atp requirement for caspase-8 and caspase-3 activation duri... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-28 |
Journal Detail:
|
Title: Fish physiology and biochemistry Volume: 36 ISSN: 1573-5168 ISO Abbreviation: Fish Physiol. Biochem. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100955049 Medline TA: Fish Physiol Biochem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1151-8 Citation Subset: IM |
Affiliation:
|
Faculty of Biosciences and Aquaculture, Bodø University College, 8049, Bodø, Norway. cmacaipang@yahoo.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Tea Weevil, Myllocerinus aurolineatus, is Attracted to Volatiles Induced by Conspecifics.
Next Document: Six-stage cascade paramagnetic mode magnetophoretic separation system for human blood samples.